Reagents and procedures for high-specificity labeling

A molecule with two pendant phenylarsine moieties according to the general structural Formula (VI):

• wherein R4, R5, R6 and R7 are each independently H, F, OR3, R3, OAc, NH2, or N(C1–C4 alkyl)2;
• or R4 with R5, or R6 together with R7, or both, form a ring;
• wherein R3 is H, CH(OH)CH2OH or (CH2)q—Y, wherein q is 1–4 and Y is H, OH, NH2, SH, COOH, OAc, CONH2 or CN;
• R8 is a linear or branched optionally substituted spacer having a length of from approximately 1.5 to approximately 15 Ångstroms;
• and R9 and R10 are each independently selected from the group consisting of H and sulfonate.

A molecule with two pendant phenylarsine moieties according to the general structural Formula (VII):

• wherein R4, R5, R6 and R7 are each independently H, F, OR3, R3, OAc, NH2, N(C1–C4 alkyl)2;
• or R4 with R5, or R6 together with R7, or both, form a ring;
• wherein R3 is H, CH(OH)CH2OH or (CH2)q—Y, wherein q is 1–4, and Y is H, OH, NH2, SH, COOH, OAc, CONH2 or CN;
• and R8 is a linear or branched optionally substituted spacer having a length of from approximately 1.5 to approximately 15 Ångstroms;
• and R9 and R10 are each independently selected from the group consisting of H and sulfonate.

A molecule with two pendant phenylarsine moieties according to the general structural Formula (VIII):

• wherein R4, R5, R6 and R7 are each independently H, F, OR3, R3, OAc, NH2, N(C1–C4 alkyl)2;
• or R4 with R5, or R6 together with R7, or both, form a ring;
• wherein R3 is H, CH(OH)CH2OH, or (CH2)q—Y, wherein q is 1–4 and Y is H, OH, NH2, SH, COOH, OAc, CONH2 or CN;
• and R8 is a linear or branched optionally substituted spacer having a length of from approximately 1.5 to approximately 15 Ångstroms;
• and R9 and R10 are each independently selected from the group consisting of H and sulfonate.

A molecule with two pendant phenylarsine moieties according to the general structural Formula (IX):

• wherein R4, R5, R6 and R7 are each independently H, F, OR3, R3, OAc, NH2, N(C1–C4 alkyl)2;
• or R4 with R5, or R6 together with R7, or both, form a ring;
• wherein R3 is H, CH(OH)CH2OH or (CH2)q—Y, wherein q is 1–4 and Y is H, OH, NH2, SH, COOH, OAc, CONH2 or CN;
• R8 is a linear or branched optionally substituted spacer having a length of from approximately 1.5 to approximately 15 Ångstroms;
• R9 and R10 are each independently selected from the group consisting of H and sulfonate;
• and R11 and R12 are each independently selected from the group consisting of H, (CH2)n″SO3—, and (CH2)n″SO3H, wherein n″ is 1 or 2.

A molecule with two pendant phenylarsine moieties according to the general structural Formula (X):

• wherein R4, R5, R6 and R7 are each independently H, F, OR3, R3, OAc, NH2, N(C1–C4 alkyl)2;
• or R4 with R5, or R6 together with R7, or both, form a ring;
• wherein R3 is H, CH(OH)CH2OH, or (CH2)q—Y, wherein q is 1–4 and Y is H, OH, NH2, SH, COOH, OAc, CONH2 or CN;
• R8 is a linear or branched optionally substituted spacer having a length of from approximately 1.5 to approximately 15 Ångstroms;
• R9 and R10 are each independently selected from the group consisting of H and sulfonate;
• and R11 and R12 are each independently selected from the group consisting of H, (CH2)n″SO3—, and (CH2)n″SO3H, wherein n″ is 1 or 2.

A molecule with two pendant phenylarsine moieties according to the general structural Formula (XI):

• wherein R4, R5, R6 and R7 are each independently H, F, OR3, R3, OAc, NH2, N(C1–C4 alkyl)2;
• or R4 with R5, or R6 together with R7, or both, form a ring;
• wherein R3 is H, CH(OH)CH2OH or (CH2)q—Y, wherein q is 1–4 and Y is H, OH, NH2, SH, COOH, OAc, CONH2 or CN;
• R8 is a linear or branched optionally substituted spacer having a length of from approximately 1.5 to approximately 15 Ångstroms;
• R9 and R10 are each independently selected from the group consisting of H and sulfonate;
• and R11 and R12 are each independently selected from the group consisting of H, (CH2)n″SO3—, and (CH2)n″SO3H, wherein n″ is 1 or 2.