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US 7,141,402 B2Granted
α-Agarase and process for producing the same
AssigneeTakara Bio Inc.
InventorsJun Tomono, Yoshiko Nomura, Hiroaki Sagawa, Takeshi Sakai, Ikunoshin Kato
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Abstract
A polypeptide having an α-agarase activity, a gene encoding the polypeptide, a method for producing the polypeptide by genetic engineering and a method for producing an agarooligosaccharide using the polypeptide.
Claims4
An isolated gene encoding an α-agarase, selected from the group consisting of:
- (1) a gene encoding a polypeptide containing an amino acid sequence consisting of 749 residues from residue 177 to residue 925 in the amino acid sequence of SEQ ID NO:14;
- (2) a gene containing a nucleotide sequence consisting of 2247 bases from nucleotide 529 to nucleotide 2775 in the nucleotide sequence of SEQ ID NO:12;
- and
- (3) a gene that hybridizes to the gene of (2) in a solution containing 6×SSC (1×SSC:0.15M NaCl, 0.015 M sodium citrate, pH 7.0), 0.5% SDS, 5× Denhardt's and 100 mg/ml herring sperm DNA at 65° C.
1 more element
Dependencies are inferred from the claim text.