Microbially-expressed thermotolerant phytase for animal feed
AssigneesSyngenta Participations AG, Diversa Corporation
InventorsMichael B. Lanahan, Edward Koepf, Keith Kretz
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Abstract
The invention provides methods for making and using thermotolerant phytases, e.g., a method of using a thermotolerant phytase in feed and food processing and feed or food products comprising a thermotolerant phytase.
Claims12
An animal feed composition comprising a thermotolerant phytase which retains at least 40% activity after 30 minutes at 60° C. and has a specific activity of greater than 200 U/mg at pH 4.5 and 37° C., wherein the thermotolerant phytase is encoded by a nucleic acid molecule comprising the nucleic acid sequence depicted in SEQ ID NO:4 encompassed by the restriction enzyme sites XhoI and NotI or a conservative variant thereof,
- or wherein the thermotolerant phytase comprises the polypeptide sequence of SEQ ID NO:1 or a conservative variant thereof.
An enzyme feed additive comprising a thermotolerant phytase which retains at least 40% activity after 30 minutes at 60° C. and has a specific activity of greater than 200 U/mg at pH 4.5 and 37° C., wherein the thermotolerant phytase is encoded by a nucleic acid molecule comprising the nucleic acid sequence depicted in SEQ ID NO:4 encompassed by the restriction enzyme sites XhoI and NotI or a conservative variant thereof,
- or wherein the thermotolerant phytase comprises the polypeptide sequence of SEQ ID NO:1 or a conservative variant thereof.
Dependencies are inferred from the claim text.