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US 7,138,233 B2Granted
IN vitro sorting method
AssigneeMedical Research Council
InventorsAndrew Griffiths, Dan Tawfik
Life of this patent
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Abstract
The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising of the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having a desired activity.
Claims30
A method of identifying an expressible genetic element, comprising:
- a) expressing a genetic element of a plurality of genetic elements to produce an expressed product, wherein said plurality of genetic elements are dispersed in aqueous droplets of an emulsion;
- and
- b) separating an aqueous droplet that contains said expressed product from those aqueous droplets that do not, thereby identifying said expressed genetic element.
A method of identifying an expressible genetic element, comprising:
- a) expressing a genetic element of a plurality of genetic elements to produce an expressed product, wherein said plurality of genetic elements are dispersed in aqueous droplets of an emulsion;
- and
- b) physically associating the expressed product of step (a) with the genetic element that encodes it;
- and
- c) separating the genetic element that is physically associated with its expressed product, resulting from step (b), from genetic elements that are not associated with said expressed product, thereby identifying an expressible genetic element.
2 more elements
Dependencies are inferred from the claim text.