Method of forming a polymerized hemoglobin solution from stabilized hemoglobin
AssigneeBiopure Corporation
InventorsThomas C. Page, Jose O. Torres, William R. Light
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Abstract
A stabilized hemoglobin solution is contacted with polymerizing agent. The stabilized hemoglobin solution includes stabilized tetrameric hemoglobin. At least a portion of the stabilized tetrameric hemoglobin is polymerized by reaction with the polymerizing agent, thereby producing a polymerized hemoglobin solution. In one embodiment, the stabilized hemoglobin solution includes a filtrate formed by filtrating polymerized solution of native hemoglobin through a filter having a molecular weight cut off of about 100 kD.
Claims41
A method for forming a polymerized hemoglobin solution from a stabilized hemoglobin solution, wherein, the stabilized hemoglobin solution includes hemoglobin that has previously been exposed to glutaraldehyde and then subsequently to a reducing agent, comprising the step of:
- exposing the stabilized hemoglobin solution to glutaraldehyde to further polymerize at least a portion of the hemoglobin present in the stabilized hemoglobin solution, whereby the average molecular weight of the stabilized hemoglobin solution increases to thereby produce the polymerized hemoglobin solution.
A method for forming a polymerized hemoglobin solution from a stabilized hemoglobin solution, wherein the stabilized hemoglobin solution includes hemoglobin that has previously been exposed to glutaraldehyde, and then subsequently to a reducing agent and a physiological buffer, comprising the step of:
- exposing the stabilized hemoglobin solution to glutaraldehyde at a concentration of about 1 to about 20 grams of glutaraldehyde per kilogram of hemoglobin present in the stabilized hemoglobin solution to further polymerize at least a portion of the hemoglobin present in the stabilized hemoglobin solution, whereby the average molecular weight of the stabilized hemoglobin solution increases to thereby produce the polymerized hemoglobin solution.
A method for forming a polymerized hemoglobin solution from a stabilized hemoglobin solution, comprising the steps of:
- a) exposing a solution containing native hemoglobin to glutaraldhyde to form a hemoglobin solution containing polymerized hemoglobin;
- b) exposing the hemoglobin solution that contains polymerized hemoglobin to a reducing agent to form a stabilized hemoglobin solution;
- and
- c) further exposing the stabilized hemoglobin solution to glutaraldehyde to further polymerize at least a portion of the hemoglobin present in the stabilized hemoglobin solution, whereby the average molecular weight of the stabilized hemoglobin solution increases to thereby produce the polymerized hemoglobin solution.
Dependencies are inferred from the claim text.