Aldehyde dehydrogenase gene
AssigneeDsm IP Assets B.V.
InventorsTatsuo Hoshino, Taro Miyazaki, Teruhide Sugisawa
Life of this patent
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Abstract
The present invention relates to a novel DNA which encodes aldehyde dehydrogenase (SNDH), an expression vector containing the DNA and recombinant organisms containing said DNA. Furthermore, the present invention concerns a process for producing recombinant aldehyde dehydrogenase protein and a process for producing L-ascorbic acid (vitamin C) and/or 2-keto-L-gulonic acid (2-KGA) from L-sorbosone by using the recombinant aldehyde dehydrogenase protein or recombinant organisms containing the expression vector. Also provided is a process for the production of 2KGA with a microorganism in which the gene encoding said aldehyde dehydrogenase is disrupted.
Claims14
An isolated nucleic acid molecule encoding aldehyde dehydrogenase which comprises a polynucleotide being at least 95% identical to the nucleotide sequence of SEQ ID NO:
- 1.
An isolated nucleic acid molecule encoding aldehyde dehydrogenase which comprises a polynucleotide being at least 95% identical to the polynucleotide selected from the group consisting of (a) nucleotides 258–2084 of SEQ ID NO:
- 1, (b) nucleotides 351–2084 of SEQ ID NO:
- 1, (c) nucleotides 258–1955 of SEQ ID NO:
- 1, and (d) nucleotides 351–1955 of SEQ ID NO:
- 1.
An isolated nucleic acid molecule encoding aldehyde dehydrogenase which comprises a polynucleotide selected from the group consisting of (a) a polynucleotide encoding the polypeptide having the amino acid sequence of SEQ ID NO:
- 2, (b) a polynucleotide encoding the polypeptide consisting of amino acids 32–609 of SEQ ID NO:
- 2, (c) a polynucleotide encoding the polypeptide consisting of amino acids 1–566 of SEQ ID NO:
- 2, and (d) a polynucleotide encoding the polypeptide consisting of amino acids 32–566 of SEQ ID NO:
- 2.
A process for the production of 2-KGA from L-sorbosone comprising (a) cultivating a microorganism belonging to Gluconobacter oxydans DSM 4025 in an appropriate culture medium, wherein the gene encoding aldehyde dehydrogenase represented by SEQ ID NO:
- 2 is disrupted in said microorganism, and (b) recovering and separating 2-KGA from said culture medium.
Dependencies are inferred from the claim text.