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AssigneeHitachi High-Technologies Corporation
InventorsToshiyuki Yokosuka, Atsumu Hirabayashi, Yasushi Terui, Kinya Kobayashi, Kiyomi Yoshinari
Estimating the term…
According to the existing mass spectrometric system, whether or not the informations are sufficient for analyzing substances (particularly proteins, sugars, etc.) cannot be judged in the process of measurement. Further, it is difficult to find out isomers having just the same mass number or compounds very close in mass only from the MS data. According to this invention, whether or not the retention time in the LC (or GC) of peptide formed at the time of enzymatic decomposition of protein coincides with the predicted retention time assumed from the amino acid sequence predicted from MS2 mass spectrometry data is judged within the actual time period of measurement, and thereby the quality of MS2 mass spectrometry data (quantity of information) is judged.
A mass spectrometric system comprising a sample introduction part for introducing a sample, a sample separation part for separating the sample, a tandem mass spectrometric analysis part for measuring information concerning the retention time τ of said sample in the sample separation part, an internal data base for storing information concerning a substance and retention time τ of said substance in said sample separation part, and an evaluation part for carrying out evaluation of the structure of the sample by the use of the retention time of said sample in the sample separation part and the retention time τ which has been stored into the internal data base in the sample separation part within the time period of actual measurement.
A method for tandem mass spectrometric analysis which comprises a procedure for introducing a sample, a procedure for separating the sample, a procedure for ionizing the sample, a procedure for subjecting the sample to tandem mass spectrometric analysis, a procedure for storing the mass number of the substance constituting the sample and information concerning retention time τ of said substance obtained in the procedure for separating the sample into the internal data base, a procedure for treating the mass spectrometric data, and a procedure for evaluating the mass spectrometric analytical data within the actual time period of measurement by the use of the retention time τ stored into the internal data base in the sample separation part.
Dependencies are inferred from the claim text.