Cell surface display of proteins by recombinant host cells
AssigneeZymoGenetics, Inc.
InventorSi Lok
Life of this patent
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Abstract
Methods and vectors are described for expressing recombinant proteins on the surface of host cells. These processes and compositions provide the basis for strategies to produce a fusion protein, comprising a membrane anchor that allows extracellular attachment of the fusion protein in a type II orientation.
Claims22
An isolated nucleic acid molecule comprising (a) a eukaryotic promoter, (b) a nucleotide sequence encoding a type II signal anchor domain segment, (c) a nucleotide sequence that encodes a spacer peptide, and (d) a cloning site wherein the spacer peptide comprises SEQ ID NO:
- 1, and wherein the isolated nucleic acid molecule comprises elements (a) to (d) in a 5′ to 3′ order.
An isolated nucleic acid molecule, comprising (a) a eukaryotic promoter, (b) a nucleotide sequence encoding a type II signal anchor domain segment, (c) a nucleotide sequence that encodes a spacer peptide, and (d) a gene or gene fragment, wherein the isolated nucleic acid molecule comprises elements (a) to (d) in a 5′ to 3′ order, wherein the gene or gene fragment resides in-frame with the nucleotide sequence that encodes the type II signal anchor domain, wherein the spacer peptide comprises SEQ ID NO:
- 1, and wherein the nucleotide sequence that encodes a type II signal anchor domain segment is heterologous with respect to the gene or gene fragment.
An isolated nucleic acid molecule, consisting of (a) a eukaryotic promoter, (b) a nucleotide sequence encoding a type II signal anchor domain segment, (c) a nucleotide sequence that encodes a spacer peptide, and (d) a gene or gene fragment, wherein the isolated nucleic acid molecule comprises elements (a) to (d) in a 5′ to 3′ order, wherein the gene or gene fragment resides in-frame with the nucleotide sequence that encodes the type II signal anchor domain, wherein the spacer peptide comprises SEQ ID NO;
- 1, and wherein the nucleotide sequence that encodes a type II signal anchor domain segment is heterologous with respect to the gene or gene fragment.
Dependencies are inferred from the claim text.