Acid-labile subunit (ALS) of insulin-like growth factor binding protein complex
AssigneeCentral Sydney Area Health Service
InventorRobert Charles Baxter
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Abstract
The acid-labile sub-unit (ALS) of insulin like growth factor binding protein complex in biologically pure form is described. ALS has a molecular weight between 80–115 kd as determined by SDS polyacrylamide gel electrophoresis, run under reducing conditions; and a partial N-terminal amino acid sequence as follows: Gly AspProGlyThrProGlyGluAlaGluGlyProAlaCysProAlaAlaCys- Ala wherein the first amino acid may be Gly or Ala. Also described are methods of producing ALS, compositions containing the in-vivo IGF protein complex, methods of detecting ALS in body fluids, recombinant nucleic acid sequences encoding ALS, and expression vectors and host cells containing such nucleic acid sequences.
Claims3
A method for the in vitro preparation of the in-vivo IGF binding protein complex comprising the step of admixing together IGF, the acid-stable IGF binding protein BP-53 and ALS comprising a partial N-terminal amino acid sequence comprising X-Asp-Pro-Gly-Thr-Pro-Gly-Glu-Ala-Glu Gly Pro Ala Cys Pro Ala Ala Cys, wherein X may be Gly or Ala (SEQ ID Nos:
- 1 and 2, respectively, with SEQ ID NO:
- 1 starting with Gly and SEQ ID NO:
- 2 starting with Ala).
Dependencies are inferred from the claim text.