Functionalized polymer for oligonucleotide purification
AssigneeVarian, Inc.
InventorVipul J. Shah
Life of this patent
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Abstract
Halogenated polymers of vinyl aromatic monomers comprising styrene and divinylbenzene are provided for use in a chromatographic separation method of analytes, wherein said polymer further comprises a hydrocarbyl or halocarbyl substituent comprising from 1 to 1,000,000 carbon atoms, or combinations thereof. Methods of using the halogenated polymers are also provided. The polymers and methods are particularly useful in the separation of nucleic acids labeled with hydrophobic labels such as protecting groups, fluorophores, and the like.
Claims54
A method of separating a mixture of analytes, comprising
- (1) applying the mixture of analytes to a chromatography sorbent comprising polymer beads of aromatic vinyl monomers substituted with hydrocarbyl or halocarbyl substituents, or combinations thereof, comprising from 1 to 1,000,000 carbon atoms, wherein said aromatic vinyl monomers or said hydrocarbyl substituents or both have been functionalized by halogenation, provided that when the vinyl aromatic monomers are brominated, bromination is performed utilizing electrophilic aromatic substitution;
- and
- (2) removing polar analytes from the chromatography sorbent by a hycirophilic solvent wash.
A chromatographic method for separating labeled nucleic acids from unlabeled nucleic acids comprising:
- (1) contacting a solution comprising labeled and unlabeled nucleic acids with a chromatography sorbent comprising a halogenated polymer of aromatic vinyl monomers comprising styrene and divinylbenzene substituted with hydrocarbyl or halocarbyl substituents, or combinations thereof, comprising from 1 to 1,000,000 carbon atoms, wherein said vinyl aromatic monomers or said hydrocarbyl substituents or both have been functionalized by halogenation, provided that when the vinyl aromatic monomers are brominated, bromination is performed utilizing electrophilic aromatic substitution;
- and
- (2) eluting unlabeled nucleic acids from the chromatography sorbent with a solvent wash.
A method of separating a mixture of analytes, comprising
- (1) applying the mixture of analytes to a chromatography sorbent comprising a brominated polymer comprising aromatic monomers substituted with hydrocarbyl or halocarbyl substituents, or combinations thereof, comprising from 1 to 1,000,000 carbon atoms;
- wherein the bromine substitutions are present on the aromatic monomers of the polymer, and
- (2) removing polar analytes from the chromatography sorbent by a hydrophilic solvent wash.
A method of separating a mixture of analytes, comprising
- (1) applying the mixture of analytes to a chromatography sorbent comprising a polymer comprising aromatic vinyl monomers substituted with halocarbyl substituents comprising from 1 to 1,000,000 carbon atoms;
- and
- (2) removing polar analytes from the chromatography sorbent by a hydrophiilic solvent wash.
Dependencies are inferred from the claim text.