Methods of identifying inhibitors of GADD45 polypeptide activity, and inhibitors of such activity
AssigneeThe United States of America as represented by the Department of Health and Human Sevices
InventorsXin Wei Wang, Curtis C. Harris, Albert J. Fornace, Jr., Jill D. Coursen, Qimin Zhan
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Abstract
The present invention is directed to novel methods for assaying for modulators of GADD45 polypeptide activity. The invention also provides means to sensitize a proliferating cell to a DNA base-damaging agent by administration of novel inhibitors of GADD45 polypeptide activity. The invention further provides polypeptides which interfere with the ability of Cdc2/cyclin B1 complexes to cause a pause at the G2/M stage of the cell cycle in response to GADD45, and nucleic acids which encode such polypeptides.
Claims8
An isolated polypeptide comprising a sequence of DEDDDR (SEQ ID NO:
- 5) and having no more than about 25 amino acids at either the N-terminal or C-terminal of the sequence of DEDDDR (SEQ ID NO:
- 50), wherein the polypeptide inhibits GADD45 activity by at least 10%.
Dependencies are inferred from the claim text.