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US 7,125,723 B2Granted
Method and assay kit for evaluation of the oxidative modification of protein-containing substances
OwnerIgor Popov, Gudrun Lewininventors
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Abstract
Oxidative stress in living organisms is determined in a photochemiluminescence measuring system after separation of low-molecular weight antioxidants from protein-containing test samples that were withdrawn from these organisms using an assay kit that contains a gel chromatographic column, a photosensitizer solution, a carbonate buffer solution, and a phosphate buffer solution.
Claims18
A method for quantitative analysis of the oxidative modification of a protein-comprising substance, comprising:
- purifying said protein-comprising substance, thereby removing a low-molecular weight antioxidant and providing a purified protein-comprising substance;
- measuring the antiradical properties of said purified protein-comprising substance in a free-radical generating system;
- comparing the measured properties with a calibration curve of a calibration substance at a given concentration;
- and
- expressing the antiradical capacity of said purified protein-comprising substance as concentration units of said calibration substance.
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