Method for introducing and expressing genes in animal cells, and bacterial blebs for use in same
AssigneeUniversity of Maryland Biotechnology Institute
InventorsRobert J. Powell, David Hone
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Abstract
A method for introducing and expressing genes in animal cells, in which the animal cells are transfected with bacterial blebs containing a eukaryotic expression cassette encoding the gene. Bacterial blebs comprising a eukaryotic expression cassette, wherein the bacterial blebs are derived from gram negative bacteria.
Claims16
A method for introducing and expressing a gene in animal cells comprising infecting said animal cells with bacterial blebs isolated from gram negative bacteria, wherein said bacterial blebs contain a eukaryotic expression cassette encoding said gene to be expressed by said animal cells.
An isolated bacterial bleb from a gram-negative bacteria, wherein the bacterial bleb comprises a eukaryotic expression cassette.
Dependencies are inferred from the claim text.