Culturable mitochondrial cells with no nucleus, showing mitochondrial activity
AssigneeEisai Research Institute
InventorsKazutoshi Nakano, Shigeo Ohta
Life of this patent
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Abstract
Provided are a method of producing a culturable cell with no nucleus, showing mitochondrial activity, comprising: performing cell fusion between a nucleus-less cell having mitochondrial DNA and a mitochondrial DNA-less cultured cell derived from a cancer cell; culturing resulting cybrid cells; and recovering floating cells from obtained cultured cells, and a cell obtained by the method.
Claims5
A method of producing a culturable cell population, showing mitochondrial activity, wherein the cell population contains at least one cell with a nucleus and cells with no nucleus, and wherein 95% or more among the cell population are cells with no nucleus, comprising:
- performing cell fusion between a nucleus-less cell having mitochondrial DNA and a mitochondrial DNA-less cultured cancer cell or cell of an established cell line;
- culturing resulting cybrid cells;
- and
- recovering floating cells which separate from adherent cybrid cells during the culture thereof.
A method of producing a population of cells with no nucleus showing mitochondrial activity, comprising:
- performing cell fusion between a nucleus-less cell having mitochondrial DNA and a mitochondrial DNA-less cultured cancer cell or cell of an established cell line;
- culturing resulting cybrid cells;
- recovering floating cells which separate from adherent cybrid cells during the culture thereof;
- determining the presence or absence of a nucleus in the floating cells;
- and
- selecting cells with no nucleus.
Dependencies are inferred from the claim text.