Culture device and method for eukaryotic cell transfection
AssigneeNitto Denko Corporation
InventorsYasunobu Tanaka, Lei Yu, Shouping Ji
Life of this patent
Estimating the term…
Abstract
A cell culture device for transfecting a eukaryotic cell is disclosed. The cell culture/transfection device may be a multiwell plate or slide which has been coated with a metal salt such as CaCl2. Methods of using the cell culture device to transfect mammalian cells and/or to monitor cell transfection are described. Kits are also described which include the cell transfection device, eukaryotic cells to be transformed and nucleic acid for transformation.
Claims46
A multiwell plate for transfecting a eukaryotic cell with a biomolecule wherein the bottoms of at least some of the wells are at least partially affixed with a composition consisting essentially of a transfection agent, wherein the transfection reagent is a metal salt.
A cell culture/transfection device for transfecting a eukaryotic cell, consisting essentially of a solid surface, wherein the solid surface is coated with calcium chloride in a gel matrix, wherein the concentration of the calcium chloride in the gel matrix is 10–40 mM.
Dependencies are inferred from the claim text.