Gluconate dehydratase
AssigneeMitsui Chemicals, Inc.
InventorsHitoki Miyake, Toshifumi Yamaki, Toshihiro Oikawa, Takeshi Nakamura, Hiroki Ishibashi, Yasushi Fukuiri, Atsushi Sakuma, Hironori Komatsu, Tomoyuki Ando, Kazuhiko Togashi, Hideki Umetani
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Abstract
A novel gluconate dehydratase derived from Achromobacter xylosoxidans and a gene encoding the gluconate dehydratase are provided. By reacting the gluconate dehydratase or a transformed cell containing the gene with an aldonic acid, the corresponding 2-keto-3-deoxyaldonic acid can be efficiently produced.
Claims2
An isolated gluconate dehydratase capable of dehydrating D-gluconic acid to produce 2-keto-3-deoxy-D-gluconic acid, the gluconate dehydratase maintaining at least 95% of the enzyme activity thereof after being allowed to stand in 30 mM tris(hydroxymethyl)aminomethane buffer solution with a pH of about 8.5 containing 1 mM of sodium D-gluconate and 1 mM of magnesium chloride at 55° C. for 2 hours;
- wherein the gluconate dehydratase is defined by the amino acid sequence shown in SEQ ID No:
- 2.
Dependencies are inferred from the claim text.