Luciferase and photoprotein
AssigneeChisso Corporation
InventorSatoshi Inouye
Life of this patent
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Abstract
The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector. Further, the invention provides a method for producing the recombinant Oplophorus luciferase or the photoprotein. These proteins could be recombinantly produced by culturing the host cell or by in vitro translation system using the recombinant expression vector.
Claims23
An isolated or purified polynucleotide encoding a protein selected from the group consisting of a protein comprising residues 28–196 of SEQ ID NO:
- 2, and a protein comprising residues 40–359 of SEQ ID NO:
- 4;
- or the complete complement of said polynucleotide.
An isolated or purified that comprises nucleotides 46–633 of SEQ ID NO:
- 1 luciferase activity.
An isolated or purified polynucleotide that encodes a protein comprising residues 28–196 of SEQ ID NO:
- 2.
An isolated or purified that comprises nucleotides 79–1155 of SEQ ID NO:
- 3 activity on luciferase.
An isolated or purified polynucleotide that encodes a protein comprising residues 40–359 of SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.