Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives
AssigneeCodexis, Inc.
InventorsS. Christopher Davis, John H. Grate, David R. Gray, John M. Gruber, Gjalt W. Huisman, Steven K. Ma, Lisa M. Newman, Roger Sheldon, Li A Wang
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Abstract
The present invention provides methods and compositions for preparing 4-substituted 3-hydroxybutyric acid derivatives by halohydrin dehalogenase-catalyzed conversion of 4-halo-3-hydroxybutyric acid derivatives. The present invention further provides methods and compositions for preparing 4-halo-3-hydroxybutyric acid derivatives by ketoreductase-catalyzed conversion of 4-halo-3-ketobutyric acid derivatives.
Claims34
A method for producing a 4-cyano-3-hydroxybutyric acid ester from a 4-halo-3-hydroxybutyric acid ester, the method comprising:
- (a) providing a 4-halo-3-hydroxybutyric acid ester,
- wherein the halo substituent is selected from the group consisting of chlorine, bromine, and iodine;
- and
- (b) contacting the 4-halo-3-hydroxybutyric acid ester with a halohydrin dehalogenase and cyanide under conditions sufficient to form a reaction mixture for converting the 4-halo-3-hydroxybutyric acid ester to a 4-cyano-3-hydroxybutyric acid ester.
Dependencies are inferred from the claim text.