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US 7,125,690 B2Granted
Promoters showing stationary phase-specific activity in gram-positive bacteria
AssigneeTakara Bio Inc.
InventorsTomoko Shimojo, Hikaru Takakura, Kazuyori Ochiai, Kiyozo Asada, Ikunoshin Kato
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Abstract
Isolated DNAs which are DNAs having a base sequence represented by any of SEQ ID NOS: 1 to 6 in Sequence Listing or fragments thereof and showing a stationary phase-specific promoter activity in Gram-positive bacteria.
Claims2
An isolated DNA consisting of the nucleotide sequence of SEQ ID NO:
- 3 which exhibits a promoter activity that is capable of expressing a nucleic acid operably linked to the promoter only during stationary phase in a Gram-positive bacterium.
A method for producing a protein, the method comprising:
- culturing to stationary phase a Gram-positive bacterium transformed with a recombinant DNA having a nucleic acid encoding a protein operably linked to the nucleotide sequence of SEQ ID NO:
- 3, which exhibits promoter activity, so that the nucleic acid is expressed from the promoter in SEQ ID NO:
- 3 only during stationary phase in the Gram-positive bacterium;
- and
- collecting from the resulting culture a protein expressed from the nucleic acid.
2 more elements
Dependencies are inferred from the claim text.