Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
AssigneeE. I. Du Pont de Nemours and Company
InventorsStephen K. Picataggio, Quinn Qun Zhu
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Abstract
The present invention relates to fatty acid desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to ETA; eicosapentaenoic acid (EPA) to docosapentaenoic acid (DPA); and arachidonic acid (ARA) to EPA. Nucleic acid sequences encoding codon-optimized desaturases and elongases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the codon-optimized desaturase or elongases, and recombinant host microorganisms expressing increased levels of desaturase or elongase are described.
Claims3
An isolated nucleic acid molecule selected from the group consisting of:
- a) an isolated nucleic acid molecule encoding a Δ17 desaturase enzyme as set forth in SEQ ID NO:62;
- or
- b) an isolated nucleic acid molecule that is completely complementary to (a).
Dependencies are inferred from the claim text.