Ligand (ACT-4-L) to a receptor on the surface of activated CD4+T-cells
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsWayne Godfrey, Edgar G. Engleman
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Abstract
The invention provides ligands and fragments thereof to a receptor on the surface of activated CD4+ T-cells. An exemplary ligand is designated ACT-4-L-h-1. Preferred fragments include purified extracellular domains of ligands. The invention also provides humanized and human antibodies to the ligand. The invention further provides methods of using the ligand and the antibodies in treatment of diseases and conditions of the immune system. The invention also provides methods of monitoring activated CD4+ T-cells using the ligands or fragments thereof.
Claims14
A method of detecting the presence of activated CD4+ T-cells in a sample, comprising:
- contacting a sample nucleic acid molecule obtained from a sample with a polynucleotide probe under stringent hybridization conditions of about 1×SSC and 0.1% SDS at 50° C.–70° C., wherein said polynucleotide probe comprises a nucleic acid sequence comprising nucleotides 15 through 845 of SEQ ID NO:
- 1 or a complete complement of said nucleic acid sequence, which nucleic acid sequence encodes an ACT-4 receptor polypeptide or variant thereof that is capable of specifically binding to the extracellular domain of the ACT-4-L-h-1 ligand;
- and
- detecting hybridization between said sample nucleic acid molecule and said polynucleotide probe, wherein the detection of hybridization is diagnostic of the presence of activated CD4+ T-cells in the sample.
A method of detecting the presence of activated CD4+ T-cells in a sample, comprising:
- contacting a sample nucleic acid molecule obtained from a sample with a polynucleotide probe under stringent hybridization conditions of about 1×SSC and 0.1% SDS at 50° C.–70° C., wherein said polynucleotide probe comprises a nucleic acid sequence of SEQ ID NO:
- 1 or a complete complement of said nucleic acid sequence or a fragment of at least about 15 contiguous nucleotides of said nucleic acid sequence or said complete complement, further wherein said polynucleotide probe does not hybridize under said stringent conditions to a nucleic acid sequence encoding OX-40 receptor;
- and
- detecting hybridization between said sample nucleic acid molecule and said polynucleotide probe, wherein the detection of hybridization is diagnostic of the presence of activated CD4 T-cells in the sample.
Dependencies are inferred from the claim text.