Solid-phase immobilization of proteins and peptides
AssigneeCompound Therapeutics, Inc.
InventorMarkus Kurz
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods for immobilizing a peptide or protein on a solid support. The method generally includes the following steps: (a) providing one or more templates attached to a solid support, wherein the one or more templates include (i) an RNA encoding a peptide and (ii) a peptide acceptor-linker linked to the RNA; and (b) subjecting the one or more templates to conditions that support translation and attachment of said peptide to said peptide acceptor, thereby synthesizing the one or more peptides on the solid support. Also disclosed herein are solid supports having at least one RNA-protein fusion component immobilized thereon, methods for generating protein arrays, and methods for screening molecules using these arrays.
Claims8
A solid support having at least one RNA-protein fusion component immobilized thereon, wherein said RNA-protein fusion component comprises a protein covalently bonded at its C-terminus to a peptide acceptor, wherein said peptide acceptor is joined through a linker to the RNA which encodes the protein, wherein the linker comprises a non-nucleotide moiety, said RNA-protein fusion component being attached to the solid support by the interaction of said linker and said solid support.
Dependencies are inferred from the claim text.