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AssigneeThe General Hospital Corporation
InventorsRobert Eugene Burgeson, Manuel Koch, Douglas R. Keene, William Joseph Brunken, Leena Bruckner-Tuderman
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The invention provides isolated nucleic acids molecules, designated collagen XXII nucleic acid molecules, which encode a novel collagen. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing collagen XXII nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a collagen XXII gene has been introduced or disrupted. The invention still further provides isolated collagen XXII proteins, fusion proteins, antigenic peptides and anti-collagen XXII antibodies. Diagnostic methods utilizing compositions of the invention are also provided.
An isolated nucleic acid molecule comprising a nucleotide sequence that is at least 80% identical to the nucleotide sequence of SEQ ID NO:3.
A nucleic acid comprising a nucleotide sequence at least 85% identical to an alternatively spliced variant of SEQ ID NO:1 or SEQ ID NO:3, which variant lacks the sequence corresponding to nucleotides 3458–3518 of SEQ ID NO:1.
An isolated nucleic acid consisting of the sequence of SEQ ID NO:1 or SEQ ID NO:3 and its complement.
An isolated nucleic acid that encodes a polypeptide having an amino acid sequence which is at least 80% identical to the amino acid sequence of SEQ ID NO:2.
An isolated nucleic acid that encodes a polypeptide comprising one or more of:
An isolated nucleic acid comprising a nucleotide sequence more than 500 nucleotides in length that hybridizes under highly stringent conditions, comprising 6×SSC at 45° C., followed by one wash in 0.2×SSC, 0.1% SDS at 65° C., to a nucleotide sequence consisting of SEQ ID NO:1 or 3, or the complement of SEQ ID NO:1 or 3, wherein the nucleic acid encodes a polypeptide comprising one or more of the following activities:
An isolated nucleic acid comprising a nucleotide sequence more than 500 nucleotides in length that hybridizes under highly stringent conditions, comprising 6×SSC at 45° C., followed by one wash in 0.2×SSC, 0.1% SDS at 65° C., to a nucleotide sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:2, or its complement.
An isolated probe or primer that hybridizes under highly stringent conditions, comprising 6×SSC at 45° C., followed by one wash in 0.2×SSC, 0.1% SDS at 65° C., to the sense or antisense nucleic acid sequence of SEQ ID NO:1 or SEQ ID NO:3, wherein the isolated probe or primer is between 50 and 300 nucleotides in length.
An isolated nucleic acid that comprises a nucleic acid sequence that encodes a polypeptide comprising an amino acid sequence at least 95% identical to a sequence selected from the group consisting of:
Dependencies are inferred from the claim text.