Method for high yield purification of immune globulins from blood plasma and blood plasma intermediates
AssigneeHemacare Corporation
InventorsJoshua Levy, Fred Rothstein, Bahman Shimiaei
Life of this patent
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Abstract
The method for immune serum globulin purification relates to the purification of immune globulins from blood plasma with a high degree of efficiency and a high rate of recovery. The immune globulin source is Cohn's fraction I+II+III or II+III prepared from plasma or plasma intermediates by precipitation of the paste at pH 6.7 to 6.8 in the presence of 20% ethanol and 80% purified water. A glycine extraction is followed by an anion exchange chromatography column step to achieve a significantly high yield and high purity of the concentrated protein.
Claims10
A method for producing a high yield of purified immune globulins, comprising:
- suspending immune globulins in an ethanol solution;
- adjusting the pH of the suspension to about 5.7 to 5.8;
- incubating the suspension for at least two hours;
- adding a solution of glycine, ethanol and water to the suspension;
- adjusting the pH of the suspension to about 5.2 to 5.4;
- extracting the immune globulins from the suspension in a liquid phase to provide an extract containing immune globulins;
- concentrating the extract containing immune globulins, and removing any impurities from the extract containing immune globulins using an anion exchange chromatography column to provide a purified extract solution containing immune globulins;
- concentrating the purified extract solution containing immune globulins to provide a concentrated purified extract solution containing immune globulins;
- treating the concentrated purified extract solution containing immune globulins with a solvent-detergent mixture to inactivate viruses, and removing the solvent-detergent mixture from the concentrated purified extract solution containing immune globulins;
- and
- formulating the concentrated purified extract solution containing immune globulins for final use.
Dependencies are inferred from the claim text.