Methods and compositions for efficient gene transfer using transcomplementary vectors
AssigneeHuman Gene Therapy Research Institute
InventorsJames N. Higginbotham, William J. Ramsey, Charles J. Link, Jr.
Life of this patent
Estimating the term…
Abstract
The invention includes a viral vector method and composition comprising transcomplementary replication incompetent viral vectors, preferably adenoviral vectors, which are cotransformed to a recipient cell. The two vectors complement each other and thus allow viral replication, in a synergistic combination which enhances both gene delivery and gene expression of genetic sequences contained within the vector.
Claims8
A composition for transforming a recipient cell comprising:
- first and second adenoviral vectors wherein said vectors are replication incompetent cotranscomplements of each other wherein said first or second vector is recombinant 1014.
A composition for transforming a recipient cell comprising:
- first and second adenoviral vectors wherein said vectors are replication incompetent cotranscomplements of each wherein said first or second vector is AVC2.TK.
A method of inducing tumor cell regression comprising:
- introducing to said tumor cell a first replication incompetent adenoviral vector, said vector including a nucleotide sequence which encodes a suicide gene, the expression of which is desired in said recipient tumor cell, and a second replication incompetent adenoviral vector, said vector comprising a suicide gene the expression of which is desired in said recipient cell, wherein said first and second adenoviral vectors are transcomplementary.
A method of inducing tumor cell regression comprising:
- introducing to said tumor cell a first replication incompetent adenoviral vector, said vector including a nucleotide sequence which encodes a thyroid sodium iodide symporter gene, the expression of which is desired in said recipient tumor cell, and a second replication incompetent adenoviral vector, said vector comprising a sodium iodide symporter gene the expression of which is desired in said recipient cell, wherein said first and second adenoviral vectors are transcomplementary, and thereafter
- exposing said tumor cells to radioactive iodide.
Dependencies are inferred from the claim text.