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US 7,125,517 B2Granted
Systems and methods for detection of analytes in biological fluids
AssigneeImmunetics, Inc.
InventorVictor A. Kovalenko
Life of this patent
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Abstract
The invention provides a heterogeneous immunoassay for detection of antibodies and antigens based on specific antigen-antibody immune complex formation with multiple antigen-bearing conjugate components. The invention further provides means for optimizing the assay format for the detection of both low and high-affinity antibodies, and provides means for quantitative detection of both antibody and the corresponding antigen present in a sample.
Claims13
A kit comprising
- a first component comprising biotin linked, through a polyethylene glycol spacer, to albumin,
- a second component comprising a biotin-binding moiety conjugated to an antigen or hapten that binds an antimicrobial antibody to be detected in a sample, and
- a third component comprising the antigen or hapten conjugated to a label.
A kit comprising
- a solid support to which is noncovalently adsorbed a first component comprising biotin linked, through a polyethylene glycol spacer, to albumin;
- a second component comprising a biotin-binding moiety conjugated to an antigen or hapten that binds an antimicrobial antibody to be detected in a sample;
- and
- a third component comprising the antigen or hapten conjugated to a label.
1 more element
Dependencies are inferred from the claim text.