Gene encoding acetolactate synthase
AssigneesKumiai Chemical Industry Co., Ltd., National Institute of Agrobiological Sciences
InventorsTsutomu Shimizu, Ishizue Nakayama, Kozo Nagayama, Atsunori Fukuda, Yoshiyuki Tanaka, Koichiro Kaku
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Abstract
The present invention provides a gene encoding ALS protein which shows a extremely high level of resistance to pyrimidinyl carboxy herbicides. The gene of the present invention encodes an acetolactate synthase protein, for instance, according to SEQ ID NO:1, where the acetolactate synthase protein has resistance to a pyrimidinyl carboxy herbicide and has acetolactate synthase activity.
Claims9
An isolated acid comprising a nucleic acid sequence encoding an acetolactate synthase protein in which the amino acid in the position corresponding to position 627 of the wild-type acetolactate synthase of SEQ ID NO:12 is isoleucine, wherein said acetolactate synthase protein has resistance to a pyrimidinyl carboxy herbicide and has acetolactate synthase activity.
A recombinant vector comprising a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence encoding an acetolactate synthase protein in which the amino acid in the position corresponding to position 627 of the wild-type acetolactate synthase of SEQ ID NO:12 is isoleucine, wherein said acetolactate synthase protein has resistance to a pyrimidinyl carboxy herbicide and has acetolactate synthase activity, and
- (b) a nucleic acid sequence encoding an acetolactate synthase protein in which the amino acid in the position corresponding to position 627 of the wild-type acetolactate synthase of SEQ ID NO:12 is isoleucine and the amino acid at the position corresponding to position 548 of the wild-type acetolactate synthase of SEQ ID NO:12 is leucine, wherein said acetolactate synthase protein has resistance to a pyrimidinyl carboxy herbicide and has acetolactate synthase activity.
A method for selecting a transformant cell having the nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence encoding an acetolactate synthase protein in which the amino acid in the position corresponding to position 627 of the wild-type acetolactate synthase of SEQ ID NO:12 is isoleucine, wherein said acetolactate synthase protein has resistance to a pyrimidinyl carboxy herbicide and has acetolactate synthase activity, and
- (b) a nucleic acid sequence encoding an acetolactate synthase protein in which the amino acid in the position corresponding to position 627 of the wild-type acetolactate synthase of SEQ ID NO:12 is isoleucine and the amino acid at the position corresponding to position 548 of the wild-type acetolactate synthase of SEQ ID NO:12 is leucine, wherein said acetolactate synthase protein has resistance to a pyrimidinyl carboxy herbicide and has acetolactate synthase activity, said method comprising:
- (a) introducing a vector comprising the nucleic said nucleic acid into a host as a selection marker;
- and
- (b) culturing the host in the presence of a pyrimidinyl carboxy herbicide.
Dependencies are inferred from the claim text.