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US 7,119,254 B2Granted
Endoglucanase gene promoter upregulated by the root-knot nematode
AssigneeNorth Carolina State University
InventorsEric L. Davis, Melissa Goellner
Life of this patent
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Abstract
The present invention relates to a root knot nematode responsive promoter isolated from Arabidopsis thaliana elongation specific endo-1, 4-beta-glucanase (cel1) gene, and a method of producing transgenic nematode and insect resistant plants and cells using cel1 promoter.
Claims22
A DNA construct which comprises, in the 5′ to 3′ direction:
- (a) a functional root knot nematode responsive promoter isolated from an Arabidopsis thaliana elongation specific endo-1,4-beta-glucanase gene;
- and
- (b) a heterologous DNA positioned downstream from said root knot nematode promoter and operatively linked therewith, said heterologous DNA encoding a product disruptive of nematode attack or an insecticidal protein.
A method of producing a root knot nematode resistant plant, comprising transforming the plant with a DNA construct comprising:
- a) a functional root knot nematode responsive promoter isolated from an Arabidopsis thaliana elongation specific endo-1, 4-beta-glucanase gene;
- and
- (b) a heterologous DNA positioned downstream from said root knot nematode promoter and operatively linked therewith, said heterologous DNA encoding a product disruptive of nematode attack.
Dependencies are inferred from the claim text.