Plant centromere compositions
AssigneeThe University of Chicago
InventorsKevin Keith, Gregory Copenhaver, Daphne Preuss
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Abstract
The present invention provides for the nucleic acid sequences of plant centromeres. This will permit construction of stably inherited recombinant DNA constructs and minichromosomes which can serve as vectors for the construction of transgenic plant and animal cells.
Claims38
A method of preparing a transgenic plant cell comprising contacting a starting plant cell with a recombinant nucleic acid construct comprising a plant centromere,
- wherein the plant centromere (i) comprises n copies of a repeated nucleotide sequence from genomic DNA of the plant, and (ii) confers an ability to segregate to daughter cells through cell division, wherein n is at least 5 and wherein the repeated nucleotide sequence is about 75 base pairs to about 210 base pairs,
- and whereby the starting plant cell is transformed with the recombinant nucleic acid construct.
A method of preparing a transgenic plant tissue comprising contacting a starting plant tissue with a recombinant nucleic acid construct comprising a plant centromere,
- wherein the plant centromere (i) comprises n copies of a repeated nucleotide sequence from genomic DNA of the plant, and (ii) confers an ability to segregate to daughter cells through cell division, wherein n is at least 5 and wherein the repeated nucleotide sequence is about 75 base pairs to about 210 base pairs,
- and whereby the starting plant tissue is transformed with the recombinant nucleic acid construct.
A method for shortening the time required to develop a new crop, the method comprising the steps of providing a plant minichromosome comprising a plant centromere, transforming a starting plant cell with the minichromosome, and generating a transgenic plant from the starting plant cell,
- wherein the plant centromere (i) comprises n copies of a repeated nucleotide sequence from genomic DNA of the plant, and (ii) confers an ability to segregate to daughter cells through cell division, wherein n is at least 5 and wherein the repeated nucleotide sequence is about 75 base pairs to about 210 base pairs,
- and the minichromosome comprises at least a structural gene.
A method of providing a vegetable crop comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a vegetable crop cell, transforming the vegetable crop cell with the plant minichromosome, and generating a vegetable crop from the transformed vegetable crop cell,
- wherein the plant centromere (i) comprises n copies of a repeated nucleotide sequence from genomic DNA of the plant, and (ii) confers an ability to segregate to daughter cells through cell division, wherein n is at least 5 and wherein the repeated nucleotide sequence is about 75 base pairs to about 210 base pairs,
- and the minichromosome comprises at least a structural gene.
A method of providing a fruit and vine crop comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a fruit and vine crop cell, transforming the fruit and vine crop cell with the plant minichromosome, and generating a fruit and vine crop from the transformed fruit and vine crop cell,
- wherein the plant centromere (i) comprises n copies of a repeated nucleotide sequence from genomic DNA of the plant, and (ii) confers an ability to segregate to daughter cells through cell division, wherein n is at least 5 and wherein the repeated nucleotide sequence is about 75 base pairs to about 210 base pairs,
- and the minichromosome comprises at least a structural gene.
A method of providing a field crop comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a field crop cell, transforming the field crop cell with the plant minichromosome, and generating a field crop from the transformed field crop cell,
- wherein the plant centromere (i) comprises n copies of a repeated nucleotide sequence from genomic DNA of the plant, and (ii) confers an ability to segregate to daughter cells through cell division, wherein n is at least 5 and wherein the repeated nucleotide sequence is about 75 base pairs to about 210 base pairs,
- and the minichromosome comprises at least a structural gene.
A method of providing a vegetable crop seed comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a vegetable crop cell, transforming the vegetable crop cell with the plant minichromosome, generating a vegetable crop from the transformed vegetable crop cell, fertilizing the vegetable crop, and collecting a seed containing a minichromosome from the fertilized vegetable crop,
- wherein the plant centromere (i) comprises n copies of a repeated nucleotide sequence from genomic DNA of the plant, and (ii) confers an ability to segregate to daughter cells through cell division, wherein n is at least 5 and wherein the repeated nucleotide sequence is about 75 base pairs to about 210 base pairs,
- and the minichromosome comprises at least a structural gene.
A method of providing a fruit and vine crop seed comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a fruit and vine crop cell, transforming the fruit and vine crop cell with the plant minichromosome, generating a fruit and vine crop from the transformed fruit and vine crop cell, fertilizing the fruit and vine crop, and collecting a seed containing a minichromosome from the fertilized fruit and vine crop,
- wherein the plant centromere (i) comprises n copies of a repeated nucleotide sequence from genomic DNA of the plant, and (ii) confers an ability to segregate to daughter cells through cell division, wherein n is at least 5 and wherein the repeated nucleotide sequence is about 75 base pairs to about 210 base pairs,
- and the minichromosome comprises at least a structural gene.
A method of providing a field crop seed comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a field crop cell, transforming the field crop cell with the plant minichromosome, generating a field crop from the transformed field crop cell, fertilizing the field crop, and collecting a seed containing a minichromosome from the fertilized field crop,
- wherein the plant centromere (i) comprises n copies of a repeated nucleotide sequence from genomic DNA of the plant, and (ii) confers an ability to segregate to daughter cells through cell division, wherein n is at least 5 and wherein the repeated nucleotide sequence is about 75 base oairs to about 210 base pairs,
- and the minichromosome comprises at least a structural gene.
A method of providing a crop seed comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a crop cell, transforming the crop cell with the plant minichromosome, generating a crop from the transformed crop cell, fertilizing the crop, and collecting a seed containing a minichromosome from the fertilized crop,
- wherein the plant centromere (i) comprises n copies of a repeated nucleotide sequence from a genomic DNA of the plant, and (ii) confers an ability to segregate to daughter cells through cell division, wherein n is at least 5 and wherein the repeated nucleotide sequence is about 75 base pairs to about 210 base pairs,
- and the minichromosome comprises at least a structural gene.
Dependencies are inferred from the claim text.