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AssigneesJong Mun Kim, Young Ki Paik
InventorsYoung Ki Paik, Pan Young Jeong
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The present invention discloses a method for effectively isolating and purifying a physiological active dauer pheromone compound having the following structural formula I related to aging and stress of C. elegans, and determination of the structure of such material. First, ethanol extract is prepared by completely concentrating a liquid medium with ethanol after culturing C. elegans on a S. basal liquid medium, and then this ethanol fraction is further extracted with ethyl acetate. Then, impurities are removed from ethyl acetate extract by a silica-gel adsorption chromatography and a pheromone extract is isolated and purified by amine column chromatographic procedures using various solvents as eluent. Finally, a pheromone compound having structural formula I is completely isolated and characterized by size-exclusion HPLC.
An isolated or purified dauer pheromone compound or its salts, represented by the following structural formula I.
A salt formed as the reaction product of 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid and an alkali or alkali earth metal salt base.
A composition for dauer inducing activity comprising a therapeutically effective amount of 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid or the salts thereof, and a pharmaceutically acceptable carrier.
A method for isolating and characterizing a dauer pheromone compound or its salts, represented by the following structural formula I, comprising the steps of:
Dependencies are inferred from the claim text.