Nucleic acid-bondable magnetic carrier and method for isolating nucleic acid using the same
AssigneeToyo Boseki Kabushiki Kaisha
InventorsHiroaki Uematsu, Katsuya Daimon, Satoko Yoshiga
Life of this patent
Estimating the term…
Abstract
A nucleic acid-bondable magnetic carrier of the present invention is a magnetic silica particle comprising a superparamagnetic metal oxide, wherein the magnetic silica particle has a specific surface of about 100 to about 800 m2/g.
Claims17
A method for isolating a nucleic acid, comprising the steps of:
- mixing a nucleic acid-bondable magnetic carrier which comprises magnetic silica particles containing a superparamagnetic metal oxide, a material containing a nucleic acid and a solution for extracting the nucleic acid so as to form a sample solution;
- separating the magnetic carrier to which the nucleic acid has been bonded from the sample solution using a magnetic field;
- and
- eluting the nucleic acid from the magnetic carrier to which the nucleic acid has been bonded;
- and
- wherein the magnetic silica particle has an average surface pore diameter of about 0.1 to about 60 nm.
A method for isolating a nucleic acid, comprising the steps of:
- mixing a nucleic acid-bondable magnetic carrier which comprises magnetic silica particles containing a superparamagnetic metal oxide, a material containing a nucleic acid and a solution for extracting the nucleic acid so as to form a sample solution;
- separating the magnetic carrier to which the nucleic acid has been bonded from the sample solution using a magnetic field, and
- eluting the nucleic acid from the magnetic carrier to which the nucleic acid has been bonded;
- and
- wherein the magnetic silica particle has a pore volume of about 0.01 to about 1.5 ml/g.
A method for isolating a nucleic acid, comprising the steps of:
- mixing a nucleic acid-bondable magnetic carrier which comprises magnetic silica particles containing a superparamagnetic metal oxide, a material containing a nucleic acid and a solution for extracting the nucleic acid so as to form a sample solution;
- separating the magnetic carrier to which the nucleic acid has been bonded from the sample solution using a magnetic field;
- and
- eluting the nucleic acid from the magnetic carrier to which the nucleic acid has been bonded;
- and
- wherein the magnetic silica particle has an average surface pore diameter of about 0.1 to about 60 nm and a pore volume of about 0.01 to about 1.5 ml/g.
A kit for isolating a nucleic acid, comprising a nucleic acid-bondable magnetic carrier which comprises magnetic silica particles and a solution for extracting the nucleic acid;
- and
- wherein the magnetic silica particle has a pore volume of about 0.01 to about 1.5 ml/g.
A kit for isolating a nucleic acid, comprising a nucleic acid-bondable magnetic carrier which comprises magnetic silica particles and a solution for extracting the nucleic acid;
- and
- wherein the magnetic silica particle has an average surface pore diameter of about 0.1 to about 60 nm and a pore volume of about 0.01 to about 1.5 ml/g.
A method for isolating a nucleic acid, comprising the steps of:
- mixing a nucleic acid-bondable magnetic carrier which comprises magnetic silica particles containing a superparamagnetic metal oxide, a material containing a nucleic acid and a solution for extracting the nucleic acid so as to form a sample solution;
- separating the magnetic carrier to which the nucleic acid has been bonded from the sample solution using a magnetic field;
- and
- eluting the nucleic acid from the magnetic carrier to which the nucleic acid has been bonded;
- and
- wherein the superparamagnetic metal oxide is iron oxide.
A kit for isolating a nucleic acid, comprising:
- a nucleic acid-bondable magnetic carrier which comprises magnetic silica particles containing a superparamagnetic metal oxide and a solution for extracting the nucleic acid;
- wherein the superparamagnetic metal oxide is iron oxide;
- wherein the magnetic silica particle is a composite of a superparamagnetic metal oxide having a surface covered with silica and an inorganic porous matrix material composed of fine silica particles;
- and
- wherein the magnetic silica particle has a pore volume of about 0.01 to about 1.5 ml/g.
Dependencies are inferred from the claim text.