US 7,119,183 B2 · Rapid immunoselection cloning method

Abstract

A simple and highly efficient method for cloning cDNAs including CD27 (SEQ ID NO:28) from mammalian expression libraries based on transient expression in mammalian host cells has been discovered. Novel expression vectors allowing highly efficient construction of mammalian cDNA libraries are disclosed. The cloning method of the invention which has been used to clone genes for cell surface antigens of human lymphocytes, has general application in gene cloning. Cell surface antigens cloned according to the present invention have been purified, and the nucleotide and amino acid sequences determined. These antigens have diagnostic and therapeutic utility in immune-mediated infections in mammals, including humans.

Claims1

Dependent claims

An isolated cDNA comprising SEQ ID NO:28.

Dependencies are inferred from the claim text.

Facts

Application

09/836,544Filed 17 Apr 2001 · division

Priority

25 Feb 1988

Published

US 2004/0072283 A115 Apr 2004

Granted

10 Oct 2006

Term ends

Not estimatedThe source records don’t support an estimate.

Classification16 codes

C07K 14/705
From animals; from humans: receptors; Cell surface antigens; Cell surface determinants
C07K 14/70578
Receptors; Cell surface antigens; Cell surface determinants: nGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
C12N 15/1034
Processes for the isolation, preparation or purification of DNA or RNA: isolating an individual clone by screening libraries
C12N 15/85
Vectors or expression systems specially adapted for eukaryotic hosts: for animal cells

Family

Application07/160,416Parent of this continuation-in-part · filed 25 Feb 1988

Application07/379,076Parent of this continuation-in-part · filed 13 Jul 1989

Application07/498,809Parent of this continuation-in-part · filed 23 Mar 1990

Application07/553,759Parent of this continuation-in-part · filed 13 Jul 1990

Application07/983,647Parent of this division · filed 1 Dec 1992

US 2004/0072283 A1Pre-grant publication · published 15 Apr 2004

US 6,218,525 B1Parent of this division

Application07/983,647Parent of this continuation-in-part

US 7,119,183 B2This patent

From the US filing history. Foreign family members aren’t listed.

Abstract

Facts

Application

09/836,544Filed 17 Apr 2001 · division

Priority

25 Feb 1988

Published

US 2004/0072283 A115 Apr 2004

Granted

10 Oct 2006

Term ends

Not estimatedThe source records don’t support an estimate.

Classification16 codes

C07K 14/705
From animals; from humans: receptors; Cell surface antigens; Cell surface determinants
C07K 14/70578
Receptors; Cell surface antigens; Cell surface determinants: nGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
C12N 15/1034
Processes for the isolation, preparation or purification of DNA or RNA: isolating an individual clone by screening libraries
C12N 15/85
Vectors or expression systems specially adapted for eukaryotic hosts: for animal cells

Family

Application07/160,416Parent of this continuation-in-part · filed 25 Feb 1988

Application07/379,076Parent of this continuation-in-part · filed 13 Jul 1989

Application07/498,809Parent of this continuation-in-part · filed 23 Mar 1990

Application07/553,759Parent of this continuation-in-part · filed 13 Jul 1990

Application07/983,647Parent of this division · filed 1 Dec 1992

US 2004/0072283 A1Pre-grant publication · published 15 Apr 2004

US 6,218,525 B1Parent of this division

Application07/983,647Parent of this continuation-in-part

US 7,119,183 B2This patent

From the US filing history. Foreign family members aren’t listed.

Rapid immunoselection cloning method

Life of this patent

Abstract

Claims1

Rapid immunoselection cloning method

Life of this patent

Abstract

Claims1