DNA sequences and peptides of human immunodeficiency virus (HIV-1)
AssigneesInstitut Pasteur, Centre National De La Recherche Scientifique
InventorsMarc Alizon, Pierre Sonigo, Cole Stewart, Oliver Danos, Simon Wain-Hobson
Life of this patent
Estimating the term…
Abstract
This invention is in the field of lymphadenopathy virus, which has been designated Human Immunodeficiency Virus Type 1 (HIV-1). This invention relates to a diagnostic means and method to detect the presence of DNA, RNA or antibodies of the lymphadenopathy retrovirus associated with the acquired immune deficiency syndrome or of the lymphadenopathy syndrome by the use of DNA fragments or the peptides encoded by said DNA fragments. The invention further relates to the DNA fragments, vectors comprising them and the proteins expressed.
Claims13
A nucleic acid of ORF-R of Human Immunodeficiency Virus Type 1 (HIV-1) comprising the sequence:
- 8250 8260 8270 8280 8290
- GA CAGGGCTTGG AAAGGATTTT GCTATAAGAT GGGTGGCAAG
- 8300 8310 8320 8330
- TGGTCAAAAA GTAGTGTGGT TGGATGGCCT ACTGTAAGGG
- 8340 8350 8360 8370
- AAAGAATGAG ACGAGCTGAG CCAGCAGCAG ATGGGGTGGG
- 8380 8390 8400 8410
- AGCAGCATCT CGAGACCTGG AAAAACATGG AGCAATCACA
- 8420 8430 8440 8450
- AGTAGCAATA CAGCAGCTAC CAATGCTGCT TGTGCCTGGC
- 8460 8470 8480 8490
- TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC
- 8500 8510 8520 8530
- ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT
- 8540 8550 8560 8570
- GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG
- 8580 8590 8600 8610
- AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA
- 8620 8630 8640 8650
- TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG
- 8660 8670 8680 8690
- CAGAACTACA CACCAGGGCC AGGGGTCAGA TATCCACTGA
- 8700 8710 8720 8730
- CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCCAGA
- 8740 8750 8760 8770
- TAAGGTAGAA GAGGCCAATA AAGGAGAGAA CACCAGCTTG
- 8780 8790 8800 8810
- TTACACCCTG TGAGCCTGCA TGGAATGGAT AGAGTGGAGG
- 8820 8830 8840 8850
- TTTGACAGCC GCCTAGCATT TCATCACGTG GCCCGAGAGC
- 8860 8870 8890 8900
- TCATCACGTG GCCCGAGAGC TGCATCCGGA GTACTTCAAG
- AACTGC,
- wherein the nucleic acid is in a eukaryotic expression vector that expresses a protein comprising the amino acid sequence:
- MGGKWSKSSVVGWPTVRERMRRAEPAADGVGAASRDLEKHGAITSSNTAAT NAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDI LDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLH PVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC.
A nucleic acid of ORF-R of Human Immunodeficiency Virus Type 1 (HIV-1) comprising the sequence:
- 8250 8260 8270 8280 8290 8300
- GA CAGGGCTTGG AAAGGATTTT GCTATAAGAT GGGTGGCAAG TGGTCAAAAA
- 8310 8320 8330 8340 8350
- GTAGTGTGGT TGGATGGCCT ACTGTAAGGG AAAGAATGAG ACGAGCTGAG
- 8360 8370 8380 8390 8400
- CCAGCAGCAG ATGGGGTGGG AGCAGCATCT CGAGACCTGG AAAAACATGG
- 8410 8420 8430 8440 8450
- AGCAATCACA AGTAGCAATA CAGCAGCTAC CAATGCTGCT TGTGCCTGGC
- 8460 8470 8480 8490 8500
- TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC ACCTCAGGTA
- 8510 8520 8530 8540 8550
- CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT
- 8560 8570 8580 8590 8600
- AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG
- 8610 8620 8630 8640 8650
- ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG
- 8660 8670 8680 8690 8700
- CAGAACTACA CACCAGGGCC AGGGGTCAGA TATCCACTGA CCTTTGGATG
- 8710 8720 8730 8740 8750
- GTGCTACAAG CTAGTACCAG TTGAGCCAGA TAAGGTAGAA GAGGCCAATA
- 8760 8770 8780 8790 8800
- AAGGAGAGAA CACCAGCTTG TTACACCCTG TGAGCCTGCA TGGAATGGAT
- 8810 8820 8830 8840 8850
- GACCCTGAGA GAGAAGTGTT AGAGTGGAGG TTTGACAGCC GCCTAGCATT
- 8860 8870 8890 8900
- TCATCACGTG GCCCGAGAGC TGCATCCGGA GTACTTCAAG AACTGC,
- wherein the nucleic acid is in a yeast expression vector that expresses a protein comprising the amino acid sequence:
- MGGKWSKSSVVGWPTVRERMRRAEPAADGVGAASRDLEKHGAITSSNTAAT NAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDI LDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLH PVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC.
A recombinant eukaryotic expression vector comprising a nucleic acid fragment of Human Immunodeficiency Virus Type 1 (HIV-1), wherein the vector expresses a protein comprising the amino acid sequence:
- MGGKWSKSSVVGWPTVRERMRRAEPAADGVGAASRDLEKHGAITSSNTAAT NAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDI LDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLH PVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC.
A recombinant mammalian expression vector comprising a nucleic acid fragment of Human Immunodeficiency Virus Type 1 (HIV-1), wherein the vector expresses a protein comprising the amino acid sequence:
- MGGKWSKSSVVGWPTVRERMRRAEPAADGVGAASRDLEKHGAITSSNTAAT NAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDI LDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLH PVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC.
A recombinant yeast expression vector comprising a nucleic acid fragment of Human Immunodeficiency Virus Type 1 (HIV-1), wherein the vector expresses a protein comprising the amino acid sequence:
- MGGKWSKSSVVGWPTVRERMRRAEPAADGVGAASRDLEKHGAITSSNTAAT NAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDI LDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLH PVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC.
A nucleic acid of ORF-R of Human Immunodeficiency Virus Type 1 (HIV-1) comprising the sequence:
- 8250 8260 8270 8280 8290 8300
- GA CAGGGCTTGG AAAGGATTTT GCTATAAGAT GGGTGGCAAG TGGTCAAAAA
- 8310 8320 8330 8340 8350
- GTAGTGTGGT TGGATGGCCT ACTGTAAGGG AAAGAATGAG ACGAGCTGAG
- 8360 8370 8380 8390 8400
- CCAGCAGCAG ATGGGGTGGG AGCAGCATCT CGAGACCTGG AAAAACATGG
- 8410 8420 8430 8440 8450
- AGCAATCACA AGTAGCAATA CAGCAGCTAC CAATGCTGCT TGTGCCTGGC
- 8460 8470 8480 8490 8500
- TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC ACCTCAGGTA
- 8510 8520 8530 8540 8550
- CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT
- 8560 8570 8580 8590 8600
- AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG
- 8610 8620 8630 8640 8650
- ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG
- 8660 8670 8680 8690 8700
- CAGAACTACA CACCAGGGCC AGGGGTCAGA TATCCACTGA CCTTTGGATG
- 8710 8720 8730 8740 8750
- GTGCTACAAG CTAGTACCAG TTGAGCCAGA TAAGGTAGAA GAGGCCAATA
- 8760 8770 8780 8790 8800
- AAGGAGAGAA CACCAGCTTG TTACACCCTG TGAGCCTGCA TGGAATGGAT
- 8810 8820 8830 8840 8850
- GACCCTGAGA GAGAAGTGTT AGAGTGGAGG TTTGACAGCC GCCTAGCATT
- 8860 8870 8890 8900
- TCATCACGTG GCCCGAGAGC TGCATCCGGA GTACTTCAAG AACTGC,
- wherein the sequence is linked to a promoter in a eukaryotic expression vector that allows the expression of a protein comprising the amino acid sequence:
- MGGKWSKSSVVGWPTVRERMRRAEPAADGVGAASRDLEKHGAITSSNTAAT NAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDI LDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLH PVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC.
An isolated eukaryotic expression vector that expresses Nef protein of Human Immunodeficiency Virus Type 1 (HIV-1), wherein the sequence hybridizes under stringent conditions to a DNA comprising the sequence:
- 8250 8260 8270 8280 8290 8300
- GA CAGGGCTTGG AAAGGATTTT GCTATAAGAT GGGTGGCAAG TGGTCAAAAA
- 8310 8320 8330 8340 8350
- GTAGTGTGGT TGGATGGCCT ACTGTAAGGG AAAGAATGAG ACGAGCTGAG
- 8360 8370 8380 8390 8400
- CCAGCAGCAG ATGGGGTGGG AGCAGCATCT CGAGACCTGG AAAAACATGG
- 8410 8420 8430 8440 8450
- AGCAATCACA AGTAGCAATA CAGCAGCTAC CAATGCTGCT TGTGCCTGGC
- 8460 8470 8480 8490 8500
- TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC ACCTCAGGTA
- 8510 8520 8530 8540 8550
- CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT
- 8560 8570 8580 8590 8600
- AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG
- 8610 8620 8630 8640 8650
- ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG
- 8660 8670 8680 8690 8700
- CAGAACTACA CACCAGGGCC AGGGGTCAGA TATCCACTGA CCTTTGGATG
- 8710 8720 8730 8740 8750
- GTGCTACAAG CTAGTACCAG TTGAGCCAGA TAAGGTAGAA GAGGCCAATA
- 8760 8770 8780 8790 8800
- AAGGAGAGAA CACCAGCTTG TTACACCCTG TGAGCCTGCA TGGAATGGAT
- 8810 8820 8830 8840 8850
- GACCCTGAGA GAGAAGTGTT AGAGTGGAGG TTTGACAGCC GCCTAGCATT
- 8860 8870 8890 8900
- TCATCACGTG GCCCGAGAGC TGCATCCGGA GTACTTCAAG AACTGC.
An isolated eukaryotic expression vector that encodes the following amino acid sequence:
- MGGKWSKSSVVGWPTVRERMRRAEPAADGVGAASRDLEKHGAITSSNTAATNAACA WLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWI YHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLHPVSL HGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC.
A method of making a eukaryotic expression vector that encodes the following amino acid sequence:
- MGGKWSKSSVVGWPTVRERMRRAEPAADGVGAASRDLEKHGAITSSNTAATNAACA WLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWI YHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLHPVSL HGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC
- comprising replicating the eukaryotic expression vector in a host cell.
Dependencies are inferred from the claim text.