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US 7,119,145 B2Granted
Surface graft modified resins and formation thereof
AssigneePhenomenex, Inc.
InventorsArthur R. Dixon, Emmet E. Welch, Shahana Huq
Life of this patent
Estimating the term…
Abstract
Pre-formed polymer particles are chemically modified by free radical-initiated surface graft modification. The resulting resin is specifically adapted to provide enhanced chromatographic performance throughout chromatographic separations.
Claims33
A method for making a polymeric chromatographic support comprising the steps of:
- a. selecting a plurality of polymer particles having a specified physical structure and morphology formed from at least one first monomer possessing from 10% to 100% cross-linking wherein said cross-linking is formed exclusively from monomers having at least 2 alkene groups and wherein a portion of said alkene groups remain unreacted, said polymer particles further defining a surface area ranging from about 1 to 1200 square meters per gram of the polymer particles, the particles having from 0.01 μmol/m2 to 8 μmol/m2 un-reacted alkene groups on a surface thereof;
- b. selecting a chemical modifier comprising a second monomer having free alkene groups which provide chromatographic performance, said second monomer being dissimilar from said at least one first monomer;
- c. selecting a solvent in which the chemical modifier is soluble and which is not reactive with the selected polymer particle;
- d. selecting a free radical initiator;
- e. placing the polymer particles, free radical initiator and modifier in the solvent and elevating the temperature as needed to cause a solution-based surface graft modification reaction that attaches the modifier to said unreacted alkene groups of the polymer particle without affecting said physical structure and morphology of said polymer particles.
2 more elements
A method for making a polymeric chromatographic support comprising the steps of:
- a. selecting a polymer membrane having a specified physical structure and morphology formed from at least one first monomer possessing from 10% to 100% cross-linking wherein said cross-linking is formed exclusively from monomers having at least 2 alkene groups and wherein a portion of said alkene groups remain unreacted, said polymer particles further defining a surface area ranging from about 1 to 1200 square meters per gram of the membrane, the membrane having a number of from 0.01 μmol/m2 to 8 μmol/m2 un-reacted alkene groups on a surface thereof;
- b. selecting a chemical modifier comprising a second monomer having free alkene groups which provide chromatographic performance, said second monomer being dissimilar from said at least one first monomer;
- c. selecting a solvent in which the chemical modifier is soluble and which is not reactive with the selected polymer membrane;
- d. selecting a free radical initiator;
- e. placing the polymer particles, free radical initiator and modifier in the solvent and elevating the temperature as needed to cause a solution-based surface graft modification reaction that attaches the modifier to the unreacted alkene groups of the polymer membrane without affecting said physical structure and morphology of said polymer particles.
2 more elements
A method for making a polymeric chromatographic support comprising the steps of:
- a. selecting a cylindrical polymer monolith having a specified physical structure and morphology formed from at least one first monomer possessing from 10% to 100% cross-linking wherein said cross-linking is formed exclusively from monomers having at least 2 alkene groups and wherein a portion of said alkene groups remain unreacted, said polymer particles further defining a surface area ranging from about 1 to 1200 square meters per gram of the cylindrical polymer monolith, the monolith having from 0.01 μmol/m2 to 8 μmol/m2 un-reacted alkene groups on a surface thereof;
- b. selecting a chemical modifier comprising a second monomer having free alkene groups which provide chromatographic performance said second monomer being dissimilar from said at least one first monomer;
- c. selecting a substantially pure solvent in which the chemical modifier is soluble and which is not reactive with the selected cylindrical polymer monolith;
- d. selecting a free radical initiator;
- e. placing the polymer particles, free radical initiator and modifier in the solvent and elevating the temperature as needed to cause a solution-based surface graft modification reaction that attaches the modifier to the unreacted alkene groups of the cylindrical polymer monolith without affecting said physical structure and morphology of said polymer particles.
2 more elements
A resin comprising:
- a. a polymer particle having a specified physical structure and morphology formed from at least one first monomer possessing from 10% to 100% cross-linking wherein said cross-linking is formed exclusively from monomers having at least 2 alkene groups and wherein a portion of said alkene groups remain unreacted, said polymer particles further and defining a surface having from 0.01 μmol/m2 to 8 μmol/m2 unreacted alkene groups formed thereon;
- and
- b. a second monomer having at least one unreacted alkene group thereon, said second monomer being dissimilar from said at least one first monomer, attached to a respective one of the unreacted alkene group on the surface of the polymer particle through surface graft modification performed in a solution that dissolves the monomer but does not chemically react with the monomer or the particle in order to form the resin, the second monomer being selected to enhance the chromatographic property of the polymer particle when attached to the unreacted alkene groups of the particle to form the resin without affecting said physical structure and morphology of said polymer particles.
Dependencies are inferred from the claim text.