Use of colostrinin, constituent peptides thereof, and analogs thereof as modulators of intracellular signaling molecules
AssigneeBoard of Regents, The University of Texas System
InventorsIstvan Boldogh, G. John Stanton, Jerzy A. Georgiades, Thomas K. Hughes, Jr., Marian Kruzel
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Abstract
The present invention provides methods that utilize compositions containing colostrinin, a constituent peptide thereof, an active analog thereof, and combinations thereof, as modulators of intracellular signaling molecules, for example.
Claims7
A method of modulating an intracellular signaling molecule in a cell, the method comprising contacting the cell with an effective amount of a modulator selected from the group consisting of colostrinin, a constituent peptide of colostrinin, and combinations thereof, under conditions effective to accomplish at least one of the following:
- reduce 4-hydroxynonenal (4HNE)-protein adduct formation;
- inhibit 4HNE-mediated glutathione depletion;
- inhibit 4HNE-induced activation of p53 protein;
- or
- inhibit 4HNE-induced activation of c-Jun NH2-tenninal kinases;
- wherein the constituent peptide of colostrinin is selected from the group consisting of MQPPPLP (SEQ ID NO:1) LQTPQPLLQVMMEPQGD (SEQ ID NO:2), DQPPDVEKPDLQPFQVQS (SEQ ID NO:3), LFFFLPVVNVLP (SEQ ID NO:4), DLEMPVLPVEPFPFV (SEQ ID NO:5), MPQNFYKLPQM (SEQ ID NO:6), VLEMKFPPPPQETVT (SEQ ID NO:7), and LKPFPLKVEVFPFP (SEQ ID NO:8).
A method of down regulating the 4-hydroxynonenal (4HNE)-mediated oxidative damage associated with lipid peroxidation in a cell, the method comprising contacting the cell with an effective amount of a modulator selected from the group consisting of colostrinin, a constituent peptide of colostrinin, and combinations thereof;
- wherein the constituent peptide of colostrinin is selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:8;
- and wherein 4HNE-mediated oxidative damage associated with lipid peroxidation in the cell is down regulated.
Dependencies are inferred from the claim text.