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US 7,118,924 B2Granted
Method for producing human antibodies in SCID mice which uses dendritic cells pulsed with antigen-antibody complexes and antigen-antibody complexes as immunizing agents
AssigneeBiogen Idec Inc.
InventorsMarco Anthony Coccia, Peter Brams
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Abstract
An improved method for producing human antibodies in SCID mice is provided. The improvement includes the use of dendritic cells pulsed with antigen-antibody complexes and antigen-antibody complexes as immunizing agents.
Claims17
A method for producing human IgG antibodies in a severe combined immunodeficiency (SCID) mouse which has been transplanted with human immune cells, which comprises
- injecting human peripheral blood lymphocytes (PBL) into a SCID mouse to produce a SCIDhu PBL mouse that contains human B cells that produces antibodies specific for an antigen with which the SCIDhu PBL mouse is immunized;
- immunizing the SCIDhu PBL mouse with antibody-antigen complex;
- and
- immunizing the SCIDhu PBL mouse with human dendritic cells which have been contacted (pulsed) in vitro with the antigen-antibody complex.
1 more element
Dependencies are inferred from the claim text.