Expression vector containing urea cycle enzyme gene, transformant thereof, and use of transformant for protein over-expression
AssigneeChung Ang University Industry Academic Cooperation Foundation
InventorsHong-Jin Kim, Myung-II Chung, Ik-Hwan Kim, Ick-Young Kim
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Abstract
The present invention relates to expression vectors containing in vivo urea cycle enzyme gene; transformants thereof; and the use of transformants for protein overexpression. During the overexpression of protein, if the animal cell transformed with expression vectors of the present invention is used, amounts of ammonia accumulation within cell culture medium will decrease and cell growth rate will increase, thus, it is advantageous to be capable of obtaining desired protein in high yields.
Claims10
An expression vector pCIneo-CPS45 (KCTC 0846BP) containing a rat CPS I gene for transforming an animal cell to be used in over-expression of proteins.
An expression vector pREP4-OTC1 (KCTC 0847BP) containing a rat OTC gene for transforming an animal cell to be used in over-expression of proteins.
An isolated CHO (Chinese Hamster Ovary) cell transformed with both an expression vector, pCIneo-CPS45 (KCTC 0846BP) containing a CPS I (carbamoyl phosphate synthetase I) gene and an expression vector, pREP4-OTC1 (KCTC 0847BP) containing an OTC (ornithine transcarbamoylase) gene.
An isolated CHO cell simultaneously transformed with an expression vector, pCIneo-CPS45 (KCTC 0846BP) containing a CPS I gene, an expression vector, pREP4-OTC1 (KCTC 0847BP) containing an OTC gene, and an expression vector, pc3.1/Zeo(+)-AS (KCTC 10014BP) containing an AS (arginosuccinate synthetase) gene.
An isolated CHO cell simultaneously transformed with an expression vector, pCIneo-CPS45 (KCTC 0846BP) containing a CPS I gene, an expression vector, pREP4-OTC1 (KCTC 0847BP) containing an OTC (ornithine transcarbamoylase) gene, an expression vector, pc3.1/Zeo(+)-AS (KCTC 10014BP) containing an AS gene, and an expression vector, pc3.1/Zeo(+)-AL (KCTC 10013BP) containing an AL (arginosuccinate lyase) gene.
An isolated CHO cell simultaneously transformed with an expression vector, pCIneo-CPS45 (KCTC 0846BP) containing a CPS I gene, an expression vector, pREP4-OTC1 (KCTC 0847BP) containing an OTC gene, an expression vector, pc3.1/Zeo(+)-AS (KCTC 10014BP) containing an AS gene, an expression vector, pc3.1/Zeo(+) -AL (KCTC 10013BP) containing an AL gene, and an expression vector, pc3. 1/Zeo(+)-Arg (KCTC 10012BP) containing an ARG (arginase) gene.
Dependencies are inferred from the claim text.