DNA molecules stabilized for propagation in bacterial cells that encode cystic fibrosis transmembrane conductance regulator
AssigneeGenzyme Corporation
InventorsRichard Gregory, Seng H. Cheng, Alan Smith, Sucharita Paul, Kathleen M. Hehir, John Marshall
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Abstract
Disclosed are full length isolated DNAs encoding cystic fibrosis transmembrane conductance regulator (CFTR) protein and a variety of mutants thereof. Also disclosed are antibodies specific for various CFTR domains and methods for their production. Expression of CFTR from cells transformed with these CFTR genes or cDNAs demonstrate surprising CFTR intracellular distributions and results thereby providing for new diagnostic and therapeutic procedures.
Claims12
A recombinant vector comprising a DNA regulatory element operably linked to a DNA molecule that encodes a wild-type human cystic fibrosis transmembrane conductance regulator protein, wherein the DNA molecule is capable of stable propagation in E. coli.
A recombinant vector comprising a DNA regulatory element operably linked to a DNA molecule encoding the cystic fibrosis transmembrane conductance regulator protein of FIG. 15 wherein the DNA molecule is capable of stable propagation in E. coli as a result of:
- (a) said DNA regulatory element permitting maintenance of the DNA molecule in E. coli at a low copy number, or
- (b) the nucleotide sequence of the DNA molecule being modified to disrupt its expression in E. coli while allowing its expression in mammalian cells.
A DNA molecule comprising:
- (a) a DNA sequence that encodes wild-type human cystic fibrosis transmembrane conductance regulator protein, and
- (b) at least one regulatory element operably linked to said DNA sequence which element permits transcription of the DNA sequence in a host prokaryotic cell.
A DNA molecule comprising:
- (a) an uninterrupted DNA sequence that encodes wild-type, human cystic fibrosis transmembrane conductance regulator protein, and
- (b) at least one regulatory element operably linked to said uninterrupted DNA sequence which element permits transcription of the uninterrupted DNA sequence in a host eukaryotic cell.
A viral vector containing an encoding sequence for human CFTR.
A viable host E. coli cell that comprises a DNA sequence coding for human CFTR protein.
Dependencies are inferred from the claim text.