On-spot selectively activated hydrophobic slide and preparation thereof

An on-spot selectively activated hydrophobic microarray, comprising:

• a substrate;
• a hydrophobic copolymer layer coated on the substrate;
• wherein the hydrophobic copolymer layer comprises spots containing a functionally active copolymer having at least a functional group capable of forming a covalent bond with a biologically active material;
• wherein the spots are separated by an inactive copolymer which is inert to react with the biologically active material;
• wherein the functionally active copolymer comprises a hydrophobic material and a compound bearing a functional group which is imide or cyclic amide, and the inactive copolymer comprises a hydrophobic material and a compound bearing the functional group protected by a protecting group, wherein the functional group is imide or cyclic amide, and the protecting group is a photo acid group;
• wherein the functionally active copolymer is formed by removing the protecting group from the functional group of the inactive copolymer;
• and
• wherein the biologically active material comprises a nucleophile immobilized to the functional active copolymer via a ring-opening reaction between the functional group of the functionally active copolymer and the nucleophile of the biologically active material.