Modified ADAMTS4 molecules and method of use thereof
AssigneeWyeth
InventorsChristopher John Corcoran, Carl R. Flannery, Weilan Zeng, Lisa A. Racie, Thomas McDonagh, Bethany A. Freeman, Katy E. Georgiadis, Edward R. LaVallie
Life of this patent
Estimating the term…
Abstract
The present invention relates to modified ADAMTS4 proteins having improved stability comparing to the corresponding native, unmodified proteins. The modified ADAMTS4 proteins can be expressed and isolated in large quantities, thus allowing further characterization of the proteins, such as crystallographic and enzyme kinetic studies. The purified, stable proteins would also facilitate the production of anti-ADAMTS antibodies and the development of inhibitors to ADAMTS enzymes.
Claims14
An isolated, modified, human ADAMTS4 protein comprising
- (a) an ADAMTS4 catalytic domain wherein the glutamate at the position corresponding to position 362 of SEQ ID NO:1 is substituted with a glutamine, and
- (b) a disintegrin domain.
Dependencies are inferred from the claim text.