Protein tyrosine kinase substrate LAT and its use in the indentification of (ant)agonists of the kinase
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsLawrence E. Samelson, Weiguo Zhang
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Abstract
The invention generally relates to compositions and methods for identifying and testing tyrosine kinase signaling pathway agonists and antagonists, and more particularly, methods and compositions for screening compounds and identifying compounds that will modulate the interaction of protein tyrosine kinase substrates with their intracellular ligands, as well as between their intracellular ligands and other members of the signaling pathway.
Claims12
A method of making a purified antibody which specifically binds to the LAT polypeptide comprising an amino acid sequence according to SEQ ID NO:
- 4 comprising:
- a) immunizing an animal with a polypeptide antigen comprising at least about five amino acids of the amino acid sequence of SEQ ID NO:
- 4;
- and
- b) isolating the antibody from said animal.
A method of making a purified antibody which specifically binds to the LAT polypeptide comprising an amino acid sequence according to SEQ ID NO:
- 4 comprising:
- a) immunizing an animal with a polypeptide antigen comprising at least about five amino acids of the amino acid sequence of SEQ ID NO:
- 4
- b) isolating antibody-producing cells from the animal;
- c) fusing the antibody producing cells with immortalized cells in culture to form antibody-producing cells;
- d) culturing the cells;
- and
- c) isolating from the culture, antibodies which bind specifically to the LAT polypeptide comprising an amino acid sequence according to SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.