Gene expression vaccine
AssigneesUniversity of South Florida Board of Trustees, The Johns Hopkins University
InventorsShyam S. Mohapatra, Mukesh Kumar, Shua-ku Huang, Kam Leong
Life of this patent
Estimating the term…
Abstract
An effective prophylactic mucosal gene expression vaccine (GXV), made up of a cocktail of at least 4 different plasmid DNAs encoding corresponding RSV antigens, coacervated with chitosan to formulate nanospheres. In a murine model of RSV infection, intranasal administration with GXV results in significant induction of RSV-specific antibodies, nasal IgA antibodies, cytotoxic T lymphocytes, and IFN-γ production in the lung and splenocytes. A single dose of GXV induces a drastic reduction of viral titers.
Claims9
An immunogenic composition comprising nanospheres, wherein said nanospheres comprise plasmid DNA coacervated with chitosan, wherein said plasmid DNA encode respiratory syncytial virus (RSV) antigens M2, F, G, M, SH, NS1, NS2, N, and P, and wherein said plasmid DNA are expressed in vivo, thereby producing each of said RSV antigens.
An immunogenic composition comprising nanospheres, wherein said nanospheres comprise plasmid DNA coacervated with chitosan, wherein said plasmid DNA encode respiratory syncytial virus (RSV) antigens M2, F, G, M, SH, NS1, NS2, N, and P, wherein said immunogenic composition is an inhalant, and wherein said plasmid DNA are expressed in vivo, thereby producing each of said RSV antigens.
Dependencies are inferred from the claim text.