Method for controlling metallophosphate precipitation in high cell density fermentations
AssigneeAmgen Inc.
InventorsCraig Eric Curless, Jeffrey Burke Baclaski
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Abstract
The invention relates to the use of a phosphate glass as a phosphorus source in the media of high cell density bacterial fermentation processes in order to reduce metallophosphate precipitation reactions. The methods of the invention demonstrate a practical method for controlling levels of metallophosphate precipitation in both the nutrient media and the fermentor which also result in increased cell densities and are useful in a variety of high cell density bacterial fermentation processes.
Claims12
A method for reducing precipitation in a bacterial fermentation process for producing a recombinant protein comprising inclusion of phosphate glasses as a phosphorus source in the nutrient media during production of said protein;
- wherein said process is a high cell density fermentation process.
A method for increasing cell density in a bacterial fermentation process for producing a recombinant protein comprising inclusion of phosphate glasses as the phosphorus source in the nutrient media during production of said protein;
- wherein said process is a high cell density fermentation process.
A method for reducing precipitation in a high cell density fermentation process for producing a recombinant protein in E. coli, comprising inclusion of a phosphate glass having a chain length of about ñ=4 to about ñ=20, wherein the chain length is defined as PO4—(PO3)ñ—PO4, as a phosphorus source in the nutrient media during production of said protein.
Dependencies are inferred from the claim text.