Process for the preparation of gallic acid by co-culture
AssigneeIndian Institute of Technology an Indian Institute of Kharagpur
InventorsRintu Banerjee, Gargi Mukherjee
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Abstract
A process for the preparation of gallic acid using co-culture comprising providing a tannin-rich mixed substrate and a culture medium in fluid communication with each other, adding an induced innoculum comprising the fungi Rhizopus oryzae and Aspergillus foetidus to the substrate to obtain a fermented mass and gallic acid, extracting the gallic acid from the culture medium and the fermented mass using an organic solvent followed by evaporation of the organic solvent to obtain gallic acid.
Claims15
A process for the preparation of gallic acid comprising:
- providing a tannin-rich mixed substrate and a culture medium, in a liquid medium;
- adding an induced innoculum comprising the fungi Rhizopus oryzae and Aspergillus foetidus to said substrate to obtain a fermented mass and gallic acid;
- extracting gallic acid from the culture medium and the fermented mass using an organic solvent;
- and
- evaporating the organic solvent to obtain gallic acid.
A process for the preparation of gallic acid comprising:
- providing a tannin-rich mixed substrate and a culture medium, in a liquid medium, wherein said tannin-rich mixed substrate comprises myrabalan fruit powder (Terminalia chebula) and gallo seed cover powder (Caesalpinia digyna) in a proportion of 1:3 to 1:⅓;
- adding an induced innoculum comprising the fungi Rhizopus oryzae and Aspergilus foetidus to said substrate to obtain a fermented mass and gallic acid, wherein the ratio of the fungi Rhizopus oryzae and Aspergillus foetidus ranges from 0.25:1 to 1:1;
- extracting gallic acid from the culture medium and the fermented mass using an organic solvent;
- and
- evaporating the organic solvent to obtain gallic acid.
Dependencies are inferred from the claim text.