Method for increasing luminescence assay sensitivity

A method for increasing the sensitivity of a luminescent assay comprising carrying out the assay in the presence of an organic compound that reduces luminescence generated by luminogenic molecules not bound to an enzyme by at least about 10 fold, and that reduces the luminescence generated by luminogenic molecules bound to an enzyme by less than about 7 fold, wherein the organic compound has at least one carbon-sulfur single bond (C—S), carbon-sulfur double bond (C═S), or carbon-selenium double bond (C═Se).

A method for increasing the sensitivity of a bio-luminescent assay comprising carrying out the assay in the presence of an organic compound that reduces autoluminescence by at least about 10 fold, and that reduces luminescence that is dependent on the presence of an analyte by less than about 7 fold, wherein the organic compound has at least one carbon-sulfur single bond (C—S), carbon-sulfur double bond (C═S), or carbon-selenium double bond (C═Se).

An assay kit comprising packaging material containing 1) a luminogenic substrate of a luminescent enzyme, or a luminogenic enzyme;

• and 2) an organic compound in an amount for reducing luminescence that is not dependent on the presence of an analyte by at least about 10 fold, and for reducing luminescence that is dependent on the presence of an analyte by less than about 7 fold, wherein the organic compound has at least one carbon-sulfur single bond (C—S), carbon-sulfur double bond (C═S), or carbon-selenium double bond (C═Se).

An assay kit comprising packaging material containing 1) a luminogenic substrate of a luminescent enzyme, or a luminogenic enzyme;

• and 2) an organic compound for reducing luminescence generated by luminogenic molecules not bound to an enzyme by at least about 10 fold, and for reducing luminescence generated by luminogenic molecules bound to an enzyme by less than about 7 fold, wherein the organic compound has at least one carbon-sulfur single bond (C—S), carbon-sulfur double bond (C═S), or carbon-selenium double bond (C═Se).

An assay kit comprising 1) a luminogenic substrate of a luminescent enzyme, or a luminogenic enzyme;

• and 2) an organic compound in an amount for reducing autoluminescence by at least about 10 fold, and for reducing luminescence that is dependent on the presence of an analyte by less than about 7 fold, wherein the organic compound has at least one carbon-sulfur single bond (C—S), carbon-sulfur double bond (C═S), or carbon-selenium double bond (C═Se).

A method for increasing the sensitivity of a bio-luminescent assay comprising carrying out the assay in the presence of an organic compound that reduces the luminescence that does not result from a bio-luminescent reaction by at least about 10 fold, and that reduces luminescence that does result from a bio-luminescent reaction by less than about 7 fold, wherein the organic compound has at least one carbon-sulfur single bond (C—S), carbon-sulfur double bond (C═S), or carbon-selenium double bond (C═Se).

An assay kit comprising packaging material containing 1) a luminogenic substrate of an enzyme, or a luminogenic enzyme;

• and 2) an organic compound for reducing luminescence that does not result from a bio-luminescent reaction by at least about 10 fold, and that reduces luminescence does result from a bio-luminescent reaction by less than about 7 fold, wherein the organic compound has at least one carbon-sulfur single bond (C—S), carbon-sulfur double bond (C═S), or carbon-selenium double bond (C═Se).