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US 7,118,866 B2Granted
Heterodimeric receptor libraries using phagemids
AssigneeThe Scripps Research Institute
InventorsAngray Kang, Carlos F. Barbas, III, Richard A. Lerner
Life of this patent
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Abstract
Filamentous phage comprising a matrix of cpVIII proteins encapsulating a genome encoding first and second polypeptides of an antogenously assembling receptor, such as an antibody, and a receptor comprised of the first and second polypeptides surface-integrated into the matrix via a filamentous phage coat protein membrane anchor domain fused to at least one of the polypeptides.
Claims5
A method for detecting the presence of a preselected ligand in a sample comprising the steps of:
- a) admixing a sample suspected to contain a preselected ligand with a ligand-binding heterodimeric antibody comprising first and second polypeptides wherein said first polypeptide is fused to a filamentous phage membrane cpVIII anchor domain, wherein said ligand-binding heterodimeric antibody binds to said preselected ligand under binding conditions sufficient for said ligand-binding heterodimeric antibody to bind said ligand and form a ligand-antibody complex;
- and
- b) detecting the presence of said ligand-antibody complex or said ligand-binding heterodimeric antibody in said complex.
Dependencies are inferred from the claim text.