D-enzyme compositions and methods of their use
AssigneeThe Scripps Research Institute
InventorsStephen Brian Henry Kent, Saskia Charlotte Florence Milton, Raymond Cecil deLisle Milton
Life of this patent
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Abstract
D-enzyme compositions are described comprising an amino acid residue sequence that defines an polypeptide able to catalyze an enzymatic reaction. The D-enzyme has an amino acid residue sequence consisting essentially of D-amino acids.
Claims12
A method for screening a library for identifying an active enantiomer of a chiral drug candidate having activity with respect to an L-protein, wherein the activity is selected from the group consisting of inhibition and binding, the method comprising the following steps:
- Step A:
- providing a D-protein, the D-protein being an enantiomer of the L-protein, and a chemical library comprising chiral compounds;
- Step B:
- contacting one or more compounds from the library under suitable conditions with the D-protein of said Step A and identifying at least one compound having activity to said D-protein;
- Step C:
- providing one or more enantiomer compounds of said at least one compound identified in Step B, and contacting said L-protein with said one or more said enantiomer compounds;
- and
- Step D:
- identifying at least one enantiomer compound provided in Step C that binds to said L-protein in order to verify the activity of said enantiomer compound that binds to said L-protein.
A method for screening a library for identifying an enantiomer compound that binds to an L-protein, said method comprising:
- Step A:
- contacting one or more compounds from a chemical library with a D-protein, wherein said D-protein is an enantiomer of said L-protein;
- Step B:
- identifying at least one compound that binds to said D-protein;
- Step C:
- providing one or more enantiomer compounds of said at least one compound identified in Step B, and contacting said L-protein with said one or more enantiomer compounds;
- and
- Step D:
- identifying at least one enantiomer compound provided in Step C that binds to said L-protein.
A method for screening a library for identifying an enantiomer compound that binds to an L-protein, said method comprising:
- Step A:
- contacting one or more compounds from a chemical library with both said L-protein and a D-protein, wherein said D-protein is an enantiomer of said L-protein;
- Step B:
- identifying at least one compound that binds to said L-protein and/or to said D-protein;
- and
- Step C:
- optionally, if said at least one compound identified in Step B binds to said D-protein, then providing one or more enantiomer compounds of said at least one compound identified in Step B, and contacting said L-protein with said one or more enantiomer compounds and identifying at least one enantiomer compound that binds to said L-protein.
Dependencies are inferred from the claim text.