Compositions and methods for inhibiting the proliferation and invasiveness of malignant cells comprising E-domain peptides of IGF-I
AssigneeUniversity of Connecticut
InventorsThomas T. Chen, Maria J. M. Chen, Ya-Huei Kuo
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Abstract
A method is described for using an a-type or b-type E domain of the human or trout IGF peptide for the inhibition of proliferation and invasiveness of a broad spectrum of malignant cells and for the inhibition of angiogenesis. The peptide species can be a homologue of the E-domain of IGF-1 or a fusion protein comprising the E-domain of IGF-1. It can be administered to one or more malignant cells in a pharmaceutically acceptable composition, or alternatively, can be administered to one or more malignant cells by transforming the cells with exogenous nucleic acid that results in the expression of the E domain of IGF-1 in the cell.
Claims44
A method for inhibiting proliferation of malignant cells, comprising administering en effective amount to one or more malignant cells of at least one of an E-domain insulin-like growth factor (IGF-I) peptide selected from the group consisting of Ea-2 domain peptide having the amino acid sequence of SEQ ID NO:
- 4, Ea-4 domain peptide having the amino acid sequence of SEQ ID NO:2, and hEb domain having the amino acid sequence of SEQ ID NO:1.
A method for reducing invasiveness of malignant cells, comprising administering an effective amount to one or more malignant cells of at least one of an E-domain insulin-like growth factor (IGF-I) peptide selected from the group consisting of Ea-2 domain peptide having the amino acid sequence of SEQ ID NO:4, Ea-4 domain peptide having the amino acid sequence of SEQ ID NO:2, and hEb domain having the amino acid sequence of SEQ ID NO:1.
A method for inhibiting angiogenic activity within animal tissue, comprising administering an effective amount to one or more malignant cells of at least one of an E-domain insulin-like growth factor 1 (IGF-I) peptide selected from the group consisting of Ea-2 domain peptide having the amino acid sequence of SEQ ID NO:4, Ea-4 domain peptide having the amino acid sequence of SEQ ID NO:2, and hEb domain having the amino acid sequence of SEQ ID NO:1.
A method for inhibiting growth of a vascularized mass of malignant cells, comprising exposing the vascularized mass to an effective amount of at least one of an E-domain insulin-like growth factor 1 (IGF-I) peptide selected from the group consisting of Ea-2 domain peptide having the amino acid sequence of SEQ ID NO:4, Ea-4 domain peptide having the amino acid sequence of SEQ ID NO:2, and hEb domain having the amino acid sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.