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US 7,118,711 B1Granted
Microcolumn reactor
AssigneeClondiag Chip Technologies GmbH
InventorJohann Michael Koehler
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AssigneeClondiag Chip Technologies GmbH
InventorJohann Michael Koehler
Estimating the term…
The invention relates to a microcolumn reactor for carrying out reactions on solid phases and/or with biological cells. The aim of the invention is to provide a microcolumn reactor for processes of synthesis and separation on small sample volumina that replaces an alternating sequence of binding and elution processes on a phase that is stationary bound during a test series and makes it possible to exchange the stationary phase, for example once it has been loaded. To this end, the microcolumn reactor consists of at least one first and one second substrate wafer (1; 2) that are linked with one another across their surfaces. At least one of the substrate wafers (1; 2) is provided with an elongate channel (3) which comprises in a defined section (a) of its length two passage openings (41, 42) that are implemented in the opposite substrate wafer. Said openings (41, 42) are separated from the channel (3) by a partially permeable sieve-type membrane (5). The areas of acceptance (51) of said membrane have a diameter that is chosen in such a manner as to specifically prevent microbeads and/or cells introduced into the channel from entering the passage openings (41, 42). The channel (3) is provided with at least two further openings (61, 62) outside the section (a) that is comprised by the passage openings (41, 42). Said further openings permit the microbeads and/or cells that are provided above the section (a) to be introduced and/or displaced by applying a fluid pressure (p). Means are provided that temporarily close at least one of the passage openings (41, 42) and one of openings (61, 62).
Microcolumn reactor for carrying out reactions on solid phases and/or biological cells comprising at least a first and a second substrate wafer being engaged to one another in a common plane, whereby at least one longitudinally extending channel is inserted into at least one of said substrate wafers, said channel, in a preselectable section of its length, being captured by two passage openings, which are passed through the an opposite substrate wafer, wherein the passage openings are separated from the channel by a partially permeable sieve-like membrane, the membrane having transmission areas so dimensioned that they preselectably prevent micro-beads and/or cells, which are introduced into the channel, from entering into the passage openings, and the channel is provided with at least two further openings outside of the section captured by said passage openings, said at least two further openings being adapted to enable a loading and/or a displacement of the micro-beads and/or cells, provided above the section captured by said preselectable channel section, by applying a fluidic pressure, and further comprising means for temporarily closing at least one of the passage openings and one of the further openings.
Dependencies are inferred from the claim text.