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US 7,115,794 B2Granted
Method for direct nucleic acid uptake
AssigneeUniversity of Hawaii
InventorPiera S. Sun
Life of this patent
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Abstract
The present invention relates to a method of nucleic acid molecule delivery into a fertilized egg. This method involves providing a fertilized egg prior to its formation of a protective layer, providing a nucleic acid molecule, and combining the nucleic acid molecule and the fertilized egg under conditions effective to allow the nucleic acid molecule to be delivered into the egg.
Claims14
A method of producing a transgenic shrimp, said method comprising:
- providing a fertilized shrimp egg prior to its full formation of a protective layer;
- providing a nucleic acid molecule;
- and
- combining the nucleic acid molecule and the fertilized egg under conditions effective to allow the nucleic acid molecule to be delivered into the egg without removing the protective layer, thereby yielding a transgenic shrimp having the nucleic acid molecule integrated into the genomic DNA of said transgenic shrimp.
1 more element
Dependencies are inferred from the claim text.